ABCD4 Polyclonal antibody

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  WB analysis using 15935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15935-1-AP (ABCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ABCD4 Polyclonal Antibody for WB, ELISA

Cat No. 15935-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ABC41, ABCD4, EST352188, P70R, P79R, PMP69, PMP70 related protein, PXMP1 L, PXMP1L

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15935-1-AP targets ABCD4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8783

  Product name: Recombinant human ABCD4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 335-606 aa of BC012815

  Sequence: SDVAGYTHRIGQLRETLLDMSLKSQDCEILGESKWGLDTPPGWPAAEPADTAFLLERVSISAPSSDKPLIKDLSLKISEGQSLLITGNTGTGKTSLLRVLGGLWTSTRGSVQMLTDFGPHGVLFLPQKPFFTDGTLREQVIYPLKEVYPDSGSADDERILRFLELAGLSNLVARTEGLDQQVDWNWYDVLSPGEMQRLSFARLFYLQPKYAVLDEATSALTEEVESELYRIGQQLGMTFISVGHRQSLEKFHSLVLKLCGGGRWELMRIKVE

• Full Name: ATP-binding cassette, sub-family D (ALD), member 4
• Calculated Molecular Weight: 606 aa, 69 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC012815
• Gene Symbol: ABCD4
• Gene ID (NCBI): 5826
• RRID: AB_3085475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14678
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCD4, also known as P70R, PMP69, and PXMP1L, belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCD4 Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner (PMID: 22922874, 33845046, 28572511). ABCD4 is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. Mutations in ABCD4 cause a new inborn error of vitamin B12 metabolism, which is related to methylmalonic aciduria and homocystinuria type cblJ (MAHCJ).