ABI1 Polyclonal antibody

ABI1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27387-1-AP

Synonyms

Abelson interactor 1, ABI 1, ABI1, Abl binding protein 4, abl interactor 1, AblBP4, E3B1, Eps8 binding protein, Nap1 binding protein, NAP1BP, SSH3BP, SSH3BP1



Tested Applications

Positive WB detected inNeuro-2a cells, C2C12 cells, multi cells, HEK-293T cells, HepG2 cells, HEK 293 cells, Jurkat cells, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse skin tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

27387-1-AP targets ABI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABI1 fusion protein Ag26537
Full Name abl-interactor 1
Calculated molecular weight 507 aa, 55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC024254
Gene symbol ABI1
Gene ID (NCBI) 10006
RRIDAB_2880860
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABI1 antibody 27387-1-APDownload protocol
IHC protocol for ABI1 antibody 27387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols