ABL1 Monoclonal antibody

ABL1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E7A5

Cat no : 68254-1-Ig

Synonyms

ABL, ABL1, c ABL, JTK7, p150, Proto oncogene c Abl, Tyrosine protein kinase ABL1, v abl



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF detected inU2OS cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68254-1-Ig targets ABL1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ABL1 fusion protein Ag26152
Full Name c-abl oncogene 1, receptor tyrosine kinase
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberNM_005157
Gene Symbol ABL1
Gene ID (NCBI) 25
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABL1, also named as ABL, JTK7, c-ABL and p150, belongs to the protein kinase superfamily, Tyr protein kinase family and ABL subfamily. ABL1 regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. ABL1 localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. It regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. ABL1 catalyze the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The antibody is specific to ABL1.

Protocols

Product Specific Protocols
WB protocol for ABL1 antibody 68254-1-IgDownload protocol
IF protocol for ABL1 antibody 68254-1-IgDownload protocol
FC protocol for ABL1 antibody 68254-1-IgDownload protocol
Standard Protocols
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