ABLIM2 Polyclonal antibody

ABLIM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22433-1-AP

Synonyms

abLIM 2, ABLIM2, Actin binding LIM protein 2, KIAA1808



Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22433-1-AP is ABLIM2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABLIM2 fusion protein Ag18198
Full Name actin binding LIM protein family, member 2
Calculated molecular weight 611 aa, 68 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC122567
Gene symbol ABLIM2
Gene ID (NCBI) 84448
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABLIM2 antibody 22433-1-APDownload protocol
IHC protocol for ABLIM2 antibody 22433-1-APDownload protocol
IP protocol for ABLIM2 antibody 22433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-10-2019)

Cell lysate on the left lane expressed GFP-ABLIM2 while on the right lane was wildtype cells. Antibody recognizes the GFP tagged construct ABLIM2 really well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ABLIM2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22433-1-AP)