Aggrecan Monoclonal antibody, PBS Only

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  IHC staining of human lung cancer using 68350-1-Ig (same clone as 68350-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68350-1-Ig (ACAN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68350-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68350-1-Ig (same clone as 68350-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ACAN antibody (68350-1-Ig, Clone: 1G11B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68350-1-PBS in a different storage buffer formulation.

Aggrecan Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 68350-1-PBS

Clone No.1G11B3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

ACAN, 1G11B3, Aggrecan core protein 2, Aggrecan 1, AGCAN

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Product Information

68350-1-PBS targets Aggrecan in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4985

  Product name: Recombinant human ACAN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-298 aa of BC036445

  Sequence: MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCS

• Full Name: aggrecan
• Calculated Molecular Weight: 250 kDa
• GenBank Accession Number: BC036445
• Gene Symbol: ACAN
• Gene ID (NCBI): 176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16112
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Aggrecan (ACAN), also named CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of the extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.