Tested Applications
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68350-1-Ig targets Aggrecan in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4985 Product name: Recombinant human ACAN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC036445 Sequence: MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCS Predict reactive species |
| Full Name | aggrecan |
| Calculated Molecular Weight | 250 kDa |
| GenBank Accession Number | BC036445 |
| Gene Symbol | ACAN |
| Gene ID (NCBI) | 176 |
| RRID | AB_3085073 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16112 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Aggrecan (ACAN), also named CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of the extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Aggrecan antibody 68350-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Aggrecan Monoclonal antibody (Cat# 68350-1-Ig) has 31 total citations. These appear in journals including Advanced Materials, Bioactive Materials, ACS Nano, Journal of Advanced Research, Redox Biology, Journal of Nanobiotechnology, Advanced Science, Journal of Controlled Release, Advanced Healthcare Materials, Materials Today Bio, Free Radical Biology and Medicine, Stem Cell Research & Therapy, Small, and others. Research fields center on osteoarthritis, cartilage regeneration and repair, intervertebral disc degeneration, chondrocyte ferroptosis, and extracellular matrix biology, with applications in hydrogels, exosomes, and nanoparticle therapies.
| Species | Application | Title |
|---|---|---|
Adv Mater Mechano-Iontronic Hydrogels Generating Biomimetic Endogenous Bioelectricity for Promoting Cartilage Regeneration. | ||
Bioact Mater Bioenergetic-active hydrogel drives extracellular matrix synthesis for cartilage repair | ||
ACS Nano Microenvironment-Responsive Hydrogels Comprising Engineering Zeolitic Imidazolate Framework-8-Anchored Parathyroid Hormone-Related Peptide-1 for Osteoarthritis Therapy | ||
J Adv Res Antioxidant taurine inhibits chondrocyte ferroptosis through upregulation of OGT/Gpx4 signaling in osteoarthritis induced by anterior cruciate ligament transection | ||
J Nanobiotechnology Targeted lipid nanoparticles distributed in hydrogel treat osteoarthritis by modulating cholesterol metabolism and promoting endogenous cartilage regeneration |
