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ACE2 Polyclonal antibody

ACE2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21115-1-AP

Synonyms

ACE related carboxypeptidase, ACE2, ACEH, Angiotensin converting enzyme 2, Metalloprotease MPROT15



Tested Applications

Positive WB detected inmouse kidney tissue, human skeletal muscle tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human lung tissue, mouse testis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21115-1-AP is ACE2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACE2 fusion protein Ag15455
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight 805 aa, 92 kDa
Observed molecular weight 120 kDa, 92 kDa
GenBank accession numberBC048094
Gene symbol ACE2
Gene ID (NCBI) 59272
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

Protocols

Product Specific Protocols
WB protocol for ACE2 antibody 21115-1-APDownload protocol
IHC protocol for ACE2 antibody 21115-1-APDownload protocol
IF protocol for ACE2 antibody 21115-1-APDownload protocol
IP protocol for ACE2 antibody 21115-1-APDownload protocol
FC protocol for ACE2 antibody 21115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Small

Highly Porous Microcarriers for Minimally Invasive In Situ Skeletal Muscle Cell Delivery.

Authors - Ranjith Kumar Kankala
humanFC

EBioMedicine

Alveolar macrophage dysfunction and cytokine storm in the pathogenesis of two severe COVID-19 patients.

Authors - Chaofu Wang
humanIF

bioRxiv

Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.

Authors - Zaniar Ghazizadeh
humanWB

Cell Rep

Activation of the SARS-CoV-2 Receptor Ace2 through JAK/STAT-Dependent Enhancers during Pregnancy.

Authors - Lothar Hennighausen
humanIHC

Skeletal Radiol

Concomitant calciphylaxis and COVID-19 associated thrombotic retiform purpura.

Authors - Jessica A Rotman
humanIHC

Hum Pathol

Docked SARS CoV-2 Proteins within the Cutaneous and Subcutaneous Microvasculature and their Role in the Pathogenesis of Severe COVID-19.

Authors - Cynthia Magro