CoraLite®594-conjugated ACE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACE2 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-21115

Synonyms

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

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Validation Data Gallery

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  IF Staining of mouse kidney using CL594-21115

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite®594 ACE2 antibody (CL594-21115) at dilution of 1:200.

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  IF Staining of mouse kidney using CL594-21115

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite®594 ACE2 antibody (CL594-21115) at dilution of 1:200.

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  FC experiment of HEK-293 using CL594-21115

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ACE2 (CL594-21115)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse kidney tissue
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-21115 targets ACE2 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACE2 fusion protein Ag15455
• Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Calculated Molecular Weight: 805 aa, 92 kDa
• Observed Molecular Weight: 120 kDa, 92 kDa
• GenBank Accession Number: BC048094
• Gene Symbol: ACE2
• Gene ID (NCBI): 59272
• RRID: AB_2919858
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).