Tested Applications
| Positive WB detected in | Jurkat cells, MCF-7 cells, HSC-T6 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67166-1-Ig targets ACLY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7709 Product name: Recombinant human ACLY protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 751-1101 aa of BC006195 Sequence: MFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM Predict reactive species |
| Full Name | ATP citrate lyase |
| Calculated Molecular Weight | 121 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC006195 |
| Gene Symbol | ACLY |
| Gene ID (NCBI) | 47 |
| RRID | AB_2882462 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | P53396 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACLY antibody 67166-1-Ig | Download protocol |
| IHC protocol for ACLY antibody 67166-1-Ig | Download protocol |
| WB protocol for ACLY antibody 67166-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACLY Monoclonal antibody (Cat# 67166-1-Ig) has 25 citations published in journals including Nature Communications, Theranostics, Advanced Science, Cell Death & Disease, Cancer Letters, Phytomedicine, Regenerative Biomaterials, Antioxidants, Aging Cell, Journal of Ethnopharmacology, International Immunopharmacology, Journal of Lipid Research, iScience, and Human Molecular Genetics. Research fields span lipid metabolism, liver cancer, lung cancer, ovarian cancer, NAFLD, atherosclerosis, mitochondrial function, and epigenetic regulation.
| Species | Application | Title |
|---|---|---|
Nat Commun SREBP1-mediated lipogenesis promotes dedifferentiation and senescence of vascular smooth muscle cells through epigenetic remodeling.
| ||
Theranostics A natural small molecule isoginkgetin alleviates hypercholesterolemia and atherosclerosis by targeting ACLY | ||
Adv Sci (Weinh) Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells | ||
Cell Death Dis ARHGEF3 regulates the stability of ACLY to promote the proliferation of lung cancer | ||
Cancer Lett Mitochondrial ACSS1 Links Acetate Metabolism to Pyrimidine Biosynthesis in Nutrient-Stressed B-Cell Lymphomas. | ||
Phytomedicine Melittin suppresses ovarian cancer growth by regulating SREBP1-mediated lipid metabolism |
Reviews
What customers say
The product has one review, rated 5 stars. It was used for immunofluorescence on sheep embryonic fibroblasts at a 1:100 dilution (lot 10010577). No written comments were provided, but the high rating indicates the user was satisfied with the antibody's performance in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Domenico (Verified Customer) (10-26-2022) |
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