ACLY Monoclonal antibody

ACLY Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgM


Human, Mouse, Rat and More (1)







Cat no : 67166-1-Ig


ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67166-1-Ig targets ACLY in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen ACLY fusion protein Ag7709
Full Name ATP citrate lyase
Calculated molecular weight 121 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC006195
Gene symbol ACLY
Gene ID (NCBI) 47
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for ACLY antibody 67166-1-IgDownload protocol
IHC protocol for ACLY antibody 67166-1-IgDownload protocol
IF protocol for ACLY antibody 67166-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mediators Inflamm

The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism.

Authors - Yu Hu

Cell Death Dis

ARHGEF3 regulates the stability of ACLY to promote the proliferation of lung cancer

Authors - Feifei Zhou


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Domenico (Verified Customer) (10-26-2022)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Sheep embryonic fibroblasts