ACLY Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67166-1-Ig (same clone as 67166-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67166-1-Ig (same clone as 67166-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 67166-1-Ig (same clone as 67166-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACLY antibody (67166-1-Ig, Clone: 1E10D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ACLY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67166-1-PBS

Clone No.1E10D5

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ATP citrate lyase, 1E10D5, ACL, ATP-citrate, ATP-citrate synthase

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Product Information

67166-1-PBS targets ACLY in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7709

  Product name: Recombinant human ACLY protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 751-1101 aa of BC006195

  Sequence: MFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

• Full Name: ATP citrate lyase
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC006195
• Gene Symbol: ACLY
• Gene ID (NCBI): 47
• RRID: AB_2882462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• UNIPROT ID: P53396
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.