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smooth muscle actin Polyclonal antibody
smooth muscle actin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14395-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse colon tissue, HUVEC cells, human placenta tissue, A549 cells, mouse liver tissue, mouse kidney tissue, rat colon tissue, mouse heart tissue, rat heart tissue
|Positive IP detected in
|mouse liver tissue
|Positive IHC detected in
|human liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|C2C12 cells, H9C2 cells, mouse heart tissue, mouse small intestine tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:1500-1:6000
|IF : 1:800-1:3200
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14395-1-AP targets smooth muscle actin in WB, IP, IHC, IF, FC, competitive binding assay, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, monkey, rabbit, zebrafish, pig, canine
|Host / Isotype
|Rabbit / IgG
|smooth muscle actin fusion protein Ag5886
|actin, alpha 2, smooth muscle, aorta
|Calculated molecular weight
|377 aa, 42 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.
|Product Specific Protocols
|WB protocol for smooth muscle actin antibody 14395-1-AP
|IHC protocol for smooth muscle actin antibody 14395-1-AP
|IF protocol for smooth muscle actin antibody 14395-1-AP
|IP protocol for smooth muscle actin antibody 14395-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (09-22-2022)
Used by IF. Very well antibody.
Wu (Verified Customer) (09-08-2022)
Used by IF. Really good signal
Emma (Verified Customer) (11-29-2021)
Used by IF on FFPE prostate tissue. Tris-EDTA pH 9.0 antigen retrieval.
Ryan (Verified Customer) (02-28-2019)
Tissue was fixed in PFA and antigen retrieval was done in NaCit ph=6.