ACTA2/smooth muscle actin Antibody 83 Publications

Rabbit Polyclonal| Catalog number: 14395-1-AP

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Freight/Packing

Con: 44 μg/150 μl

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Tested Applications

Applications:
ELISA, FC, IF, IHC, IP, WB
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Species specificity:
human, mouse, rat

Positive WB detected in:
human placenta tissue, mouse kidney tissue, mouse liver tissue, rat heart tissue

Positive IP detected in:
mouse liver tissue

Positive IHC detected in:
human heart tissue, human hysteromyoma tissue, human liver cancer tissue, human liver tissue, human skeletal muscle tissue

Positive IF detected in:
C2C12 cell, mouse heart tissue

Positive FC detected in:
C2C12 cell

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:200-1:8000
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
actin, alpha 2, smooth muscle, aorta

Calculated molecular weight:
377aa,42 kDa

Observed molecular weight:
43 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ACTA2

Synonyms
a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, Actin, aortic smooth muscle Actin, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, α SMA
Background

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.


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