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  • KD/KO Validated

smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, competitive binding assay, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14395-1-AP

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, Actin, aortic smooth muscle Actin, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, smooth muscle actin, α SMA, α-sma



Tested Applications

Positive WB detected inmouse colon tissue, HUVEC cells, human placenta tissue, A549 cells, mouse liver tissue, mouse kidney tissue, rat colon tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, H9C2 cells, mouse heart tissue, mouse small intestine tissue
Positive FC detected inC2C12 cell

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:800-1:3200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14395-1-AP targets smooth muscle actin in WB, IP, IHC, IF, FC, competitive binding assay, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, rabbit, zebrafish, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen smooth muscle actin fusion protein Ag5886
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC017554
Gene symbol ACTA2
Gene ID (NCBI) 59
RRIDAB_2223009
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Protocols

Product Specific Protocols
WB protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IHC protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IF protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IP protocol for smooth muscle actin antibody 14395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Adv Mater

Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring

Authors - Huihui He
mouseIF

Mil Med Res

Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease

Authors - Guang-Hui Deng
mouseWB

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
IHC

ACS Nano

Endoscopy Deliverable and Mushroom-Cap-Inspired Hyperboloid-Shaped Drug-Laden Bioadhesive Hydrogel for Stomach Perforation Repair

Authors - Shuang Liu
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
human,ratWB

ACS Nano

Hepatic Stellate Cell- and Liver Microbiome-Specific Delivery System for Dihydrotanshinone I to Ameliorate Liver Fibrosis

Authors - Xia Niu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

James (Verified Customer) (09-22-2022)

Used by IF. Very well antibody.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: kidney
smooth muscle actin Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:14395-1-AP)
FH

Wu (Verified Customer) (09-08-2022)

Used by IF. Really good signal

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: fibroblast cell
smooth muscle actin Antibody Immunofluorescence validation (1:150 dilution) in fibroblast cell (Cat no:14395-1-AP)
FH

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EDTA pH 9.0 antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
smooth muscle actin Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:14395-1-AP)
FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA and antigen retrieval was done in NaCit ph=6.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish ocular muscles at 3dpf