human placenta tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

rat heart tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human heart tissue slide using 14395-1-AP( ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

Immunohistochemistry of paraffin-embedded human heart tissue slide using 14395-1-AP( ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).

Immunohistochemistry of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical of paraffin-embedded human hysteromyoma using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human hysteromyoma using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 14395-1-AP( ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 14395-1-AP( ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).

Immunohistochemistry of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens).

Immunohistochemistry of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens).

IP Result of anti-ACTA2/smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed C2C12 cell using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed C2C12 cell using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

1X10^6 C2C12 cell were stained with 0.2ug ACTA2/smooth muscle actin antibody (14395-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.