smooth muscle actin Polyclonal antibody
smooth muscle actin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
246
Cat no : 14395-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue, mouse kidney tissue, human placenta tissue, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | C2C12 cell, mouse heart tissue, C2C12 cells |
| Positive FC detected in | C2C12 cell |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 176 publications below |
| IHC | See 49 publications below |
| IF | See 71 publications below |
Product Information
14395-1-AP targets smooth muscle actin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | smooth muscle actin fusion protein Ag5886 |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated molecular weight | 377 aa, 42 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC017554 |
| Gene symbol | ACTA2 |
| Gene ID (NCBI) | 59 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
| IHC protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
| IF protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
| IP protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
| FC protocol for smooth muscle actin antibody 14395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Ther Med Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor-β1-mediated Smad and mitogen-activated protein kinase signaling transductions in vivo and in vitro. | ||
J Cell Physiol Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts. | ||
Aging (Albany NY) Anti-senescence role of heterozygous fumarate hydratase gene knockout in rat lung fibroblasts in vitro. | ||
Toxicol Lett The emerging roles of a novel CCCH-type zinc finger protein, ZC3H4, in silica-induced epithelial to mesenchymal transition. | ||
Antioxid Redox Signal Peroxisome proliferator-activated receptor-γ coactivator-1α inhibits vascular calcification through sirtuin 3‒mediated reduction of mitochondrial oxidative stress. | ||
Eur J Pharmacol Death-associated protein kinase 3 deficiency alleviates vascular calcification via AMPK-mediated inhibition of endoplasmic reticulum stress. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ryan (Verified Customer) (02-28-2019) | Tissue was fixed in PFA and antigen retrieval was done in NaCit ph=6.
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