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ACTA2/smooth muscle actin Antibody 125 Publications

Rabbit Polyclonal| Catalog number: 14395-1-AP

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Con: 44 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

ACTA2/smooth muscle actin Antibody IHC human liver cancer tissue 14395-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ACTA2/smooth muscle actin Antibody IHC human colon tissue 14395-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ACTA2/smooth muscle actin Antibody WB human placenta tissue 14395-1-AP

human placenta tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ACTA2/smooth muscle actin Antibody IHC human heart tissue 14395-1-AP

Immunohistochemistry of paraffin-embedded human heart tissue slide using 14395-1-AP( ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

ACTA2/smooth muscle actin Antibody IHC human heart tissue 14395-1-AP

Immunohistochemistry of paraffin-embedded human heart tissue slide using 14395-1-AP( ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).

ACTA2/smooth muscle actin Antibody IHC human liver cancer tissue 14395-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ACTA2/smooth muscle actin Antibody IF C2C12 cell 14395-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed C2C12 cell using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ACTA2/smooth muscle actin Antibody IP mouse liver tissue 14395-1-AP

IP Result of anti-ACTA2/smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

ACTA2/smooth muscle actin Antibody IF mouse heart tissue 14395-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ACTA2/smooth muscle actin Antibody IF mouse heart tissue 14395-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 14395-1-AP(ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ACTA2/smooth muscle actin Antibody FC C2C12 cell 14395-1-AP

1X10^6 C2C12 cell were stained with 0.2ug ACTA2/smooth muscle actin antibody (14395-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
human placenta tissue, mouse kidney tissue, rat heart tissue, mouse liver tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human liver cancer tissue, human colon tissue, stromal tumor tissue, human hysteromyoma tissue, human skeletal muscle tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
C2C12 cell, mouse heart tissue
Positive FC detected in:
C2C12 cell
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:1500-1:6000
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACTA2/smooth muscle actin fusion protein Ag5886
Full name:
actin, alpha 2, smooth muscle, aorta
Calculated molecular weight:
377aa,42 kDa
Observed molecular weight:
43 kDa
GenBank accession number:
BC017554
Gene ID (NCBI):
59
Gene symbol
ACTA2
Synonyms
a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, Actin, aortic smooth muscle Actin, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, α SMA

Background

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.


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