CoraLite®488-conjugated smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-14395

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA



Tested Applications

Positive IF detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14395-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-14395 targets smooth muscle actin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen smooth muscle actin fusion protein Ag5886
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC017554
Gene symbol ACTA2
Gene ID (NCBI) 59
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Protocols

Product Specific Protocols
IF protocol for CL488 smooth muscle actin antibody CL488-14395Download protocol
Standard Protocols
Click here to view our Standard Protocols