CoraLite® Plus 488-conjugated smooth muscle actin Polyclonal antibody
smooth muscle actin Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-14395
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | mouse heart tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-14395 targets smooth muscle actin in IF applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | smooth muscle actin fusion protein Ag5886 |
Full Name | actin, alpha 2, smooth muscle, aorta |
Calculated molecular weight | 377 aa, 42 kDa |
Observed molecular weight | 43 kDa |
GenBank accession number | BC017554 |
Gene symbol | ACTA2 |
Gene ID (NCBI) | 59 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 smooth muscle actin antibody CL488-14395 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |