CoraLite® Plus 488-conjugated Alpha smooth muscle actin Polyclonal antibody

• Featured Product
• KD/KO Validated

Alpha smooth muscle actin Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

4

Cat no : CL488-14395

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

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Validation Data Gallery

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  IF Staining of mouse heart using CL488-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL488-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200, CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

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Tested Applications

• Positive IF-P detected in: mouse heart tissue, mouse small intestine tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below

Product Information

CL488-14395 targets Alpha smooth muscle actin in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha smooth muscle actin fusion protein Ag5886
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 377 aa, 42 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC017554
• Gene Symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• RRID: AB_2919090
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Publications

Species	Application	Title
mouse	IF	Cell Metab; Myocardial infarction accelerates the progression of MASH by triggering immunoinflammatory response and induction of periosti; Authors - Wei Xie
mouse	IF	Front Med; Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ; Authors - Fei Xu
	IF	Diabet Med; Mouse islet-derived stellate cells are similar to, but distinct from, mesenchymal stromal cells and influence the beta cell function; Authors - Wei Xu
rat	IF	Regen Biomater; Magnetic fibrin nanofiber hydrogel delivering iron oxide magnetic nanoparticles promotes peripheral nerve regeneration; Authors - Juncong Hong