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Beta Actin Polyclonal antibody
Beta Actin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 20536-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse colon tissue, A549 cells, C6 cells, HeLa cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, rat kidney tissue, Caco-2 cells, RAW 264.7 cells, SMMC-7721 cells, Jurkat cells, rat spleen tissue, HepG2 cells, mouse brain tissue, rat brain tissue|
|Positive IHC detected in||human colon tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
20536-1-AP targets Beta Actin in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, monkey, canine samples.
|Tested Reactivity||human, mouse, rat, monkey, canine|
|Cited Reactivity||human, mouse, rat, goat, chicken, carp, yeast, sheep, Ochotona curzoniae, Camelus bactrianus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Beta Actin fusion protein Ag14521|
|Full Name||actin, beta|
|Calculated molecular weight||375 aa, 42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC002409|
|Gene ID (NCBI)||60|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Julia (Verified Customer) (06-20-2023)
Our lab has been using Proteintech's beta actin polyclonal antibody for years. We use this antibody in all our western blot experiments for normalization. This antibody works consistently at a 1:1000 dilution for human and mouse retina and for HRECs. We have always gotten a beautiful result, with nice thick and specific bands. The only downside is that this antibody is hard to strip. Even after stripping, the membrane tends to have residual beta actin bands. Even so, we would highly recommend this product for routine western blots.
Chun (Verified Customer) (06-19-2022)
This antibody worked very well.
Aaron (Verified Customer) (12-08-2020)
nice bands achieved
Wei (Verified Customer) (01-30-2020)
Strong, clear band for basal heart tissues.Good for internal control for heart diseases.
SCOTT (Verified Customer) (10-11-2019)
Used 1/1000 overnight 4 C. overnightHuman Grey matter cortex post mortem material
Dan (Verified Customer) (08-19-2019)
The antibody works
Kishor (Verified Customer) (01-30-2019)
Working well for Western Blotting (1:5000)