HRP-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, zebrafish, plant and More (5)

Applications

WB, IF, ELISA

Conjugate

HRP

CloneNo.

7D2C10

Cat no : HRP-60008

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP β actin, HRP-ACTB, HRP-Beta actin, HRP-F actin, PS1TP5BP1, β actin



Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

HRP-60008 targets Beta Actin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Reactivity human, mouse, rat, zebrafish, plant
Cited Reactivityhuman, mouse, rat, chicken, monkey, hamster, pig, canine
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name actin, beta
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002409
Gene symbol ACTB
Gene ID (NCBI) 60
RRIDAB_2819183
Conjugate HRP
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Protocols

Product Specific Protocols
WB protocol for HRP Beta Actin antibody HRP-60008Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell

Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate

Authors - Kazushige Shiraishi
WB

Nat Neurosci

Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.

Authors - Kornélia Szebényi
mouseWB

Blood

Fetal liver hepcidin secures iron stores in utero.

Authors - Lara Kämmerer
humanWB

Nat Microbiol

Adeno-associated virus 2 bound to its cellular receptor AAVR.

Authors - Ran Zhang
humanWB

Mol Cancer

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Authors - Ya Fan
WB

Acta Pharm Sin B

Targeting a cryptic allosteric site of SIRT6 with small-molecule inhibitors that inhibit the migration of pancreatic cancer cells.

Authors - Qiufen Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (10-24-2023)

Used Incubation over night at 4°C, never tested a shorter incubation, but it worked very nice.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: whole lysate in UREA made from muscles, brown fat and white fat. species: mice and naked molerats
FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon
FH

Wojciech (Verified Customer) (04-20-2023)

The beta actin works very well diluted at 1:5000. It was used on T-ALL cell lines. Loading 30 ug of whole protein lysate.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: T cells
FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 5000
  • Cell Tissue Type: Caco2, Mice colon
FH

Iru (Verified Customer) (06-07-2021)

The antibody works well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells
FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using HRP- conjugated beta actin in NSC34 cell line at dilution of 1:10000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: NSC34 cells
HRP Beta Actin Antibody Western Blot validation (1:10000 dilution) in NSC34 cells (Cat no:HRP-60008)
FH

Hasibur (Verified Customer) (08-01-2019)

Nice antibody and no need for secondary antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: Bladder Cancer
FH

Joanne (Verified Customer) (01-22-2019)

Tested using 10ug of Caco-2 cell lysate and whole membrane. A strong band with no background and a great time saver!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: caco-2 cell
HRP Beta Actin Antibody Western Blot, validation (1:5000 dilution) in caco-2 cell (Cat no:HRP-60008)
FH

Tess (Verified Customer) (11-29-2018)

Little to no background, and takes half the time.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: human colorectal cancer cell line
HRP Beta Actin Antibody Western Blot, validation (1:10,000 dilution) in human colorectal cancer cell line (Cat no:HRP-60008)