ACVRL1 Antibody

ACVRL1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

0

Cat no : 14745-1-AP

Synonyms

Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I



Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman heart tissue, human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

14745-1-AP targets ACVRL1 in FC, IHC, IP, WB, ELISA applications and shows reactivity with human samples.

Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACVRL1 fusion protein Ag6449
Full Name activin A receptor type II-like 1
Calculated molecular weight 56 kDa
Observed molecular weight 63 kDa
GenBank accession number BC042637
Gene symbol ACVRL1
Gene ID (NCBI) 94
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

Protocols

Product Specific Protocols
WB protocol for ACVRL1 antibody 14745-1-APDownload protocol
IHC protocol for ACVRL1 antibody 14745-1-APDownload protocol
IP protocol for ACVRL1 antibody 14745-1-APDownload protocol
FC protocol for ACVRL1 antibody 14745-1-APDownload protocol
Standard Protocols
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