ACVRL1 Polyclonal antibody

ACVRL1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, FC

Conjugate

Unconjugated

Publications

5

Cat no : 14745-1-AP

Synonyms

Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I

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Validation Data Gallery

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  WB analysis of human placenta using 14745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14745-1-AP

  IP Result of anti-ACVRL1 (IP:14745-1-AP, 4ug; Detection:14745-1-AP 1:300) with Jurkat cells lysate 2400ug.

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  IHC staining of human heart using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14745-1-AP (ACVRL1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14745-1-AP (ACVRL1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lymphoma using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Jurkat using 14745-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug ACVRL1 antibody (14745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human heart tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

14745-1-AP targets ACVRL1 in WB, IP, IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACVRL1 fusion protein Ag6449
• Full Name: activin A receptor type II-like 1
• Calculated molecular weight: 56 kDa
• Observed molecular weight: 63 kDa
• GenBank accession number: BC042637
• Gene symbol: ACVRL1
• Gene ID (NCBI): 94
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

Publications

Species	Application	Title
mouse,human	WB	Mol Cell; OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin.; Authors - Yesheng Fu
human	WB,FC	Nat Commun; Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils.; Authors - Yusuke Kimishima
human	WB	J Mol Cell Cardiol; BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.; Authors - Yongyun Li
human	WB,IP	Cell Biol Int; CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/ PI3K/ AKT pathway.; Authors - Houping Chen
human	IHC	J Thorac Dis; Pulmonary inflammatory myofibroblastic tumor versus IgG4-related inflammatory pseudotumor: differential diagnosis based on a case series.; Authors - Longfei Zhu

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