ACVRL1 Polyclonal antibody

ACVRL1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, FC

Conjugate

Unconjugated

Cat no : 14745-1-AP

Synonyms

Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I



Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman heart tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

14745-1-AP targets ACVRL1 in WB, IP, IHC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACVRL1 fusion protein Ag6449
Full Name activin A receptor type II-like 1
Calculated molecular weight 56 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC042637
Gene symbol ACVRL1
Gene ID (NCBI) 94
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

Protocols

Product Specific Protocols
WB protocol for ACVRL1 antibody 14745-1-APDownload protocol
IHC protocol for ACVRL1 antibody 14745-1-APDownload protocol
IP protocol for ACVRL1 antibody 14745-1-APDownload protocol
FC protocol for ACVRL1 antibody 14745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Mol Cell

OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin.

Authors - Yesheng Fu
humanWB,FC

Nat Commun

Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils.

Authors - Yusuke Kimishima
humanWB

J Mol Cell Cardiol

BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.

Authors - Yongyun Li
humanWB,IP

Cell Biol Int

CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/ PI3K/ AKT pathway.

Authors - Houping Chen
humanIHC

J Thorac Dis

Pulmonary inflammatory myofibroblastic tumor versus IgG4-related inflammatory pseudotumor: differential diagnosis based on a case series.

Authors - Longfei Zhu