ACVRL1 Polyclonal antibody

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  WB analysis of human placenta using 14745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14745-1-AP

  IP result of anti-ACVRL1 (IP:14745-1-AP, 4ug; Detection:14745-1-AP 1:300) with Jurkat cells lysate 2400ug.

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  IHC staining of human heart using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14745-1-AP (ACVRL1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lymphoma using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 14745-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug ACVRL1 Polyclonal antibody (14745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

ACVRL1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 14745-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

Activin receptor like kinase 1, Activin receptor-like kinase 1, ACVRLK1, ALK 1, ALK1

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human heart tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14745-1-AP targets ACVRL1 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6449

  Product name: Recombinant human ACVRL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 142-503 aa of BC042637

  Sequence: HVRRRQEKQRGLHSELGESSLILKASEQGDSMLGDLLDSDCTTGSGSGLPFLVQRTVARQVALVECVGKGRYGEVWRGLWHGESVAVKIFSSRDEQSWFRETEIYNTVLLRHDNILGFIASDMTSRNSSTQLWLITHYHEHGSLYDFLQRQTLEPHLALRLAVSAACGLAHLHVEIFGTQGKPAIAHRDFKSRNVLVKSNLQCCIADLGLAVMHSQGSDYLDIGNNPRVGTKRYMAPEVLDEQIRTDCFESYKWTDIWAFGLVLWEIARRTIVNGIVEDYRPPFYDVVPNDPSFEDMKKVVCVDQQTPTIPNRLAADPVLSGLAQMMRECWYPNPSARLTALRIKKTLQKISNSPEKPKVIQ

• Full Name: activin A receptor type II-like 1
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC042637
• Gene Symbol: ACVRL1
• Gene ID (NCBI): 94
• RRID: AB_2222474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P37023
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Mol Cell; OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin.; Authors - Yesheng Fu
human	WB	Nat Commun; Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils.; Authors - Yusuke Kimishima
human	WB	J Mol Cell Cardiol; BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.; Authors - Yongyun Li
human	WB,IP	Cell Biol Int; CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/ PI3K/ AKT pathway.; Authors - Houping Chen
human	IHC	J Thorac Dis; Pulmonary inflammatory myofibroblastic tumor versus IgG4-related inflammatory pseudotumor: differential diagnosis based on a case series.; Authors - Longfei Zhu
mouse	WB,IHC	Biosci Biotechnol Biochem; BMP9 functions as a negative regulator in the myogenic differentiation of primary mouse myoblasts; Authors - Xiya Lu