Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human heart tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14745-1-AP targets ACVRL1 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6449 Product name: Recombinant human ACVRL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 142-503 aa of BC042637 Sequence: HVRRRQEKQRGLHSELGESSLILKASEQGDSMLGDLLDSDCTTGSGSGLPFLVQRTVARQVALVECVGKGRYGEVWRGLWHGESVAVKIFSSRDEQSWFRETEIYNTVLLRHDNILGFIASDMTSRNSSTQLWLITHYHEHGSLYDFLQRQTLEPHLALRLAVSAACGLAHLHVEIFGTQGKPAIAHRDFKSRNVLVKSNLQCCIADLGLAVMHSQGSDYLDIGNNPRVGTKRYMAPEVLDEQIRTDCFESYKWTDIWAFGLVLWEIARRTIVNGIVEDYRPPFYDVVPNDPSFEDMKKVVCVDQQTPTIPNRLAADPVLSGLAQMMRECWYPNPSARLTALRIKKTLQKISNSPEKPKVIQ Predict reactive species |
| Full Name | activin A receptor type II-like 1 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC042637 |
| Gene Symbol | ACVRL1 |
| Gene ID (NCBI) | 94 |
| RRID | AB_2222474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37023 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ACVRL1 antibody 14745-1-AP | Download protocol |
| IHC protocol for ACVRL1 antibody 14745-1-AP | Download protocol |
| IP protocol for ACVRL1 antibody 14745-1-AP | Download protocol |
| WB protocol for ACVRL1 antibody 14745-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACVRL1 (ALK1) polyclonal antibody (Cat# 14745-1-AP) has 11 citations published in journals including Nature Communications, Molecular Cell, Arteriosclerosis, Thrombosis, and Vascular Biology, Journal of Molecular and Cellular Cardiology, Journal of Cellular and Molecular Medicine, Cell Biology International, Human Cell, Journal of Thoracic Disease, Bioscience, Biotechnology, and Biochemistry, Journal of Cellular Signalling, and Frontiers in Pharmacology. Research fields span pulmonary hypertension, angiogenesis, atherosclerosis, osteoarthritis, osteosarcoma, cervical cancer, and TGF-β/Smad signaling.
| Species | Application | Title |
|---|---|---|
Nat Commun Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils. | ||
Mol Cell OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin. | ||
Arterioscler Thromb Vasc Biol Inflammatory Cell-Derived MYDGF Attenuates Endothelial LDL Transcytosis to Protect Against Atherogenesis | ||
J Mol Cell Cardiol BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway. | ||
J Cell Mol Med Sodium Hyaluronate-PDGF Repairs Cartilage and Subchondral Bone Microenvironment via HIF-1α-VEGF-Notch and SDF-1-CXCR4 Inhibition in Osteoarthritis | ||
Cell Biol Int CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/ PI3K/ AKT pathway. |
