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ADAM21 Polyclonal antibody

ADAM21 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 21434-1-AP

Synonyms

ADAM 21, ADAM21, ADAM31

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ADAM21 Polyclonal antibody (21434-1-AP)

    IHC staining of human lung cancer using 21434-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21434-1-AP (ADAM21 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ADAM21 Polyclonal antibody (21434-1-AP)

    IHC staining of human lung cancer using 21434-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21434-1-AP (ADAM21 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21434-1-AP is ADAM21 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM21 fusion protein Ag16042
Full Name ADAM metallopeptidase domain 21
Calculated Molecular Weight 722 aa, 81 kDa
GenBank Accession NumberBC109024
Gene Symbol ADAM21
Gene ID (NCBI) 8747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM21 is also present in growing axonal tracts during postnatal development and in growing primary olfactory axons in adults. In the olfactory nerve layer, ADAM21 often, but not always, colocalizes with OMP, a marker of mature olfactory neurons, but is not colocalized with the immature marker βIII-tubulin. The exclusive expression of ADAM21 in human testis and its sequence similarity with the fertilins suggest that it is also expressed on sperm cells and involved in sperm maturation and/or fertilization(PMID:9469942).