• Featured Product
  • KD/KO Validated

ADARB1 Polyclonal antibody

ADARB1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 22248-1-AP

Synonyms

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1



Tested Applications

Positive WB detected inrat brain tissue, A431 cells
Positive IP detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22248-1-AP is ADARB1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADARB1 fusion protein Ag17743
Full Name adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
Calculated molecular weight 741 aa, 81 kDa
Observed molecular weight 70 kDa, 80 kDa
GenBank accession numberBC065545
Gene symbol ADARB1
Gene ID (NCBI) 104
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

Protocols

Product Specific Protocols
WB protocol for ADARB1 antibody 22248-1-APDownload protocol
IP protocol for ADARB1 antibody 22248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Function of low ADARB1 expression in lung adenocarcinoma.

Authors - Xiang Wang
  • KD Validated
humanWB

J Cell Mol Med

Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.

Authors - Lei Peng
mouseIF

Sci Rep

Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.

Authors - Alessia Mirra