|Positive WB detected in||rat brain tissue, A431 cells|
|Positive IP detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22248-1-AP is ADARB1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ADARB1 fusion protein Ag17743|
|Full Name||adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)|
|Calculated molecular weight||741 aa, 81 kDa|
|Observed molecular weight||70 kDa, 80 kDa|
|GenBank accession number||BC065545|
|Gene ID (NCBI)||104|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).
Function of low ADARB1 expression in lung adenocarcinoma.
Proline-arginine poly-dipeptide encoded by the C9orf72 repeat expansion inhibits adenosine deaminase acting on RNA.
J Cell Mol Med
Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.
Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.