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  • KD/KO Validated

ADARB1 Polyclonal antibody

ADARB1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22248-1-AP

Synonyms

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1



Tested Applications

Positive WB detected inA431 cells, 4T1 cells, C2C12 cells, HeLa cells, NIH/3T3 cells, HepG2 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22248-1-AP targets ADARB1 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADARB1 fusion protein Ag17743
Full Name adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
Calculated Molecular Weight 741 aa, 81 kDa
Observed Molecular Weight 74-86 kDa
GenBank Accession NumberBC065545
Gene Symbol ADARB1
Gene ID (NCBI) 104
RRIDAB_2879047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

Protocols

Product Specific Protocols
WB protocol for ADARB1 antibody 22248-1-APDownload protocol
IP protocol for ADARB1 antibody 22248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.

Authors - Shangming Tang
humanWB,RIP

Basic Res Cardiol

Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs.

Authors - Karoline E Kokot
mouseIF

Sci Rep

Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.

Authors - Alessia Mirra
mouseWB

FASEB J

Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling.

Authors - Ramani Soundararajan
  • KO Validated
humanWB

J Cell Mol Med

Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.

Authors - Lei Peng
humanWB

Front Pharmacol

Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma.

Authors - Can Lu