Tested Applications
| Positive WB detected in | L02 cells, HepG2 cells, pig liver tissue, rat liver tissue, SMMC-7721 cells, HuH-7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66939-1-Ig targets ADH1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10630 Product name: Recombinant human ADH1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-375 aa of BC033009 Sequence: MSTAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAVGICRTDDHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRVCKNPESNYCLKNDLGNPRGTLQDGTRRFTCRGKPIHHFLGTSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF Predict reactive species |
| Full Name | alcohol dehydrogenase 1B (class I), beta polypeptide |
| Calculated Molecular Weight | 375 aa, 40 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC033009 |
| Gene Symbol | ADH1B |
| Gene ID (NCBI) | 125 |
| RRID | AB_2882263 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P00325 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ADH1B antibody 66939-1-Ig | Download protocol |
| IHC protocol for ADH1B antibody 66939-1-Ig | Download protocol |
| WB protocol for ADH1B antibody 66939-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ADH1B Monoclonal antibody (1F1B4), catalog number 66939-1-Ig, has 10 total citations. These appear in journals including Cancer Communications, Journal of Translational Medicine, Journal of Ginseng Research, Oncoimmunology, Geroscience, Cell Reports Methods, Frontiers in Pharmacology, iScience, Frontiers in Genetics, and Current Cancer Drug Targets. Research fields span alcohol-associated liver disease, lung adenocarcinoma, triple-negative breast cancer, laryngeal cancer, gastric cancer, endometriosis, and skeletal myopathy.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Nicotinamide N-methyltransferase negatively regulates metastasis-promoting property of cancer-associated fibroblasts in lung adenocarcinoma | ||
J Transl Med Comprehensive analysis of the metabolomics and transcriptomics uncovers the dysregulated network and potential biomarkers of Triple Negative Breast Cancer | ||
J Ginseng Res Li-Ginseng powder protects against alcohol-induced liver injury by promoting acetaldehyde clearance and cellular homeostasis. | ||
Oncoimmunology HIF2A mediates lineage transition to aggressive phenotype of cancer-associated fibroblasts in lung cancer brain metastasis | ||
Geroscience Heavy alcohol use exacerbates skeletal myopathy in peripheral artery disease. | ||
Cell Rep Methods Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids |
