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ADH1B Monoclonal antibody
ADH1B Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66939-1-Ig
Validation Data Gallery
|Positive WB detected in||L02 cells, HepG2 cells, pig liver tissue, rat liver tissue, SMMC-7721 cells, HuH-7 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66939-1-Ig targets ADH1B in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
|Tested Reactivity||Human, Mouse, Rat, Pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||ADH1B fusion protein Ag10630|
|Full Name||alcohol dehydrogenase 1B (class I), beta polypeptide|
|Calculated molecular weight||375 aa, 40 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC033009|
|Gene ID (NCBI)||125|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).
A positive feed-forward loop between Fusobacterium nucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.
Kinsenoside Alleviates Alcoholic Liver Injury by Reducing Oxidative Stress, Inhibiting Endoplasmic Reticulum Stress, and Regulating AMPK-Dependent Autophagy.
Analysis of characteristic genes and ceRNA regulation mechanism of endometriosis based on full transcriptional sequencing