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ADH1B Monoclonal antibody
ADH1B Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66939-1-Ig
Validation Data Gallery
|Positive WB detected in||L02 cells, HepG2 cells, pig liver tissue, rat liver tissue, SMMC-7721 cells, HuH-7 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66939-1-Ig targets ADH1B in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
|Tested Reactivity||Human, Mouse, Rat, Pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||ADH1B fusion protein Ag10630|
|Full Name||alcohol dehydrogenase 1B (class I), beta polypeptide|
|Calculated molecular weight||375 aa, 40 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC033009|
|Gene ID (NCBI)||125|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).
Kinsenoside Alleviates Alcoholic Liver Injury by Reducing Oxidative Stress, Inhibiting Endoplasmic Reticulum Stress, and Regulating AMPK-Dependent Autophagy.
A positive feed-forward loop between Fusobacterium nucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.