• Featured Product
  • KD/KO Validated

ADH1B Monoclonal antibody

ADH1B Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1F1B4

Cat no : 66939-1-Ig

Synonyms

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125



Tested Applications

Positive WB detected inL02 cells, HepG2 cells, pig liver tissue, rat liver tissue, SMMC-7721 cells, HuH-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66939-1-Ig targets ADH1B in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ADH1B fusion protein Ag10630
Full Name alcohol dehydrogenase 1B (class I), beta polypeptide
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC033009
Gene symbol ADH1B
Gene ID (NCBI) 125
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

Protocols

Product Specific Protocols
WB protocol for ADH1B antibody 66939-1-IgDownload protocol
IHC protocol for ADH1B antibody 66939-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols