ADH7 Polyclonal antibody

ADH7 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23425-1-AP

Synonyms

ADH 4, ADH7, Gastric alcohol dehydrogenase, Retinol dehydrogenase

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Validation Data Gallery

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  WB analysis of HeLa using 23425-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23425-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23425-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 23425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 23425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 23425-1-AP

  Immunofluorescent analysis of HepG2 cells, using ADH7 antibody 23425-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HEK-293 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23425-1-AP is ADH7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADH7 fusion protein Ag19340
• Full Name: alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
• Calculated molecular weight: 386 aa, 41 kDa
• Observed molecular weight: 45-47 kDa
• GenBank accession number: BC131512
• Gene symbol: ADH7
• Gene ID (NCBI): 131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH7(Alcohol dehydrogenase class 4 mu/sigma chain) is also named as gastric alcohol dehydrogenase, retinol dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. The ubiquitous expression pattern of ADH7 during embryogenesis has led to the notion that the enzymatic conversion of retinol to retinal occurs more or less uniformly and plays no role in the spatial or temporal regulation of RA synthesis during embryonic development(PMID:17473173). It has 2 isoforms produced by alternative splicing and can exsit as a homodimer(PMID:18479436).