ADH7 Polyclonal antibody

ADH7 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23425-1-AP

Synonyms

ADH 4, ADH7, Gastric alcohol dehydrogenase, Retinol dehydrogenase



Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23425-1-AP is ADH7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH7 fusion protein Ag19340
Full Name alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
Calculated molecular weight 386 aa, 41 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC131512
Gene symbol ADH7
Gene ID (NCBI) 131
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADH7(Alcohol dehydrogenase class 4 mu/sigma chain) is also named as gastric alcohol dehydrogenase, retinol dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. The ubiquitous expression pattern of ADH7 during embryogenesis has led to the notion that the enzymatic conversion of retinol to retinal occurs more or less uniformly and plays no role in the spatial or temporal regulation of RA synthesis during embryonic development(PMID:17473173). It has 2 isoforms produced by alternative splicing and can exsit as a homodimer(PMID:18479436).

Protocols

Product Specific Protocols
WB protocol for ADH7 antibody 23425-1-APDownload protocol
IHC protocol for ADH7 antibody 23425-1-APDownload protocol
IF protocol for ADH7 antibody 23425-1-APDownload protocol
Standard Protocols
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