ADH7 Polyclonal antibody

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  WB analysis of HeLa using 23425-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23425-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23425-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 23425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 23425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 23425-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH7 antibody (23425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ADH7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23425-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Omega-hydroxydecanoate dehydrogenase ADH7, EC:1.1.1.66, EC:1.1.1.105, EC:1.1.1.1, All-trans-retinol dehydrogenase [NAD(+)] ADH7

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HEK-293 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

23425-1-AP targets ADH7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADH7 fusion protein Ag19340
• Full Name: alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
• Calculated Molecular Weight: 386 aa, 41 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: BC131512
• Gene Symbol: ADH7
• Gene ID (NCBI): 131
• RRID: AB_2879277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P40394
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH7(Alcohol dehydrogenase class 4 mu/sigma chain) is also named as gastric alcohol dehydrogenase, retinol dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. The ubiquitous expression pattern of ADH7 during embryogenesis has led to the notion that the enzymatic conversion of retinol to retinal occurs more or less uniformly and plays no role in the spatial or temporal regulation of RA synthesis during embryonic development(PMID:17473173). It has 2 isoforms produced by alternative splicing and can exsit as a homodimer(PMID:18479436).

Publications

Species	Application	Title
mouse	WB	Aging (Albany NY); Mettl3-mediated m6A modification plays a role in lipid metabolism disorders and progressive liver damage in mice by regulating lipid metabolism-related gene expression; Authors - Guanqi Dai