ADRB3 Polyclonal antibody

ADRB3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30713-1-AP

Synonyms

ADRB3, ADRB3R, adrenergic, beta 3 , receptor, B3AR, Beta 3 adrenergic receptor, Beta 3 adrenoceptor, Beta 3 adrenoreceptor, BETA3AR, FLJ99960

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Validation Data Gallery

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  WB analysis using 30713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30713-1-AP (ADRB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30713-1-AP targets ADRB3 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADRB3 fusion protein Ag31466
• Full Name: adrenergic, beta-3-, receptor
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: NM_000025
• Gene Symbol: ADRB3
• Gene ID (NCBI): 155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-3 adrenergic receptor(ADRB3), a member of the G-protein-coupled receptor family, is expressed mainly in adipose tissue and is thought to contribute to lipolysis and thermogenesis. In addition, the overexpression of ADRB3 has been found in several cancer types, including breast cancer, gallbladder cancer , and colorectal cancer. More recently, the involvement of ADRB3 in the metabolic reprogramming of melanoma, the promotion of immune tolerance, and the regulation of cancer differentiation have been described. (PMID: 32514619)