|Positive WB detected in||HL-60 cells, Jurkat cells|
|Positive IP detected in||HL-60 cells|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13990-1-AP targets GRK2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||GRK2 fusion protein Ag5071|
|Full Name||adrenergic, beta, receptor kinase 1|
|Calculated molecular weight||689 aa, 80 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC037963|
|Gene ID (NCBI)||156|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GRK2 (G-protein coupled receptor kinase 2) is also named as ADRBK1 (Beta-adrenergic receptor kinase 1), BARK, and BARK1. G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. GRK2 translocation from the cytoplasm to the cell membrane in response to extracellular signals is critical to the function of the protein, where GRK phosphorylation is an important component of its translocation to the membrane.(PMID:27412951)
|Product Specific Protocols|
|WB protocol for GRK2 antibody 13990-1-AP||Download protocol|
|IHC protocol for GRK2 antibody 13990-1-AP||Download protocol|
|IP protocol for GRK2 antibody 13990-1-AP||Download protocol|
|FC protocol for GRK2 antibody 13990-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells.
Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.
J Am Heart Assoc
Paroxetine Attenuates Cardiac Hypertrophy Via Blocking GRK2 and ADRB1 Interaction in Hypertension.
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia.
Paeoniflorin-6'-o-benzene sulfonate ameliorates the progression of adjuvant-induced arthritis by inhibiting the interaction between Ahr and GRK2 of fibroblast-like synoviocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Franziska (Verified Customer) (09-11-2023)
Since there are no GRK-2 antibodies specific for C.elegans, I decided to try this one which didn't work very well, there was a lot of unspecific binding however, C.elegans was not mentioned as tested reactivity.