Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human lymphoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 12 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13990-1-AP targets GRK2 in WB, IHC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRK2 fusion protein Ag5071 Predict reactive species |
| Full Name | adrenergic, beta, receptor kinase 1 |
| Calculated Molecular Weight | 689 aa, 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC037963 |
| Gene Symbol | ADRBK1 |
| Gene ID (NCBI) | 156 |
| RRID | AB_2225833 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25098 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRK2 (G-protein coupled receptor kinase 2) is also named as ADRBK1 (Beta-adrenergic receptor kinase 1), BARK, and BARK1. G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. GRK2 translocation from the cytoplasm to the cell membrane in response to extracellular signals is critical to the function of the protein, where GRK phosphorylation is an important component of its translocation to the membrane.(PMID:27412951)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRK2 antibody 13990-1-AP | Download protocol |
| IHC protocol for GRK2 antibody 13990-1-AP | Download protocol |
| IP protocol for GRK2 antibody 13990-1-AP | Download protocol |
| FC protocol for GRK2 antibody 13990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells. | ||
Nat Commun Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
| ||
J Pharm Anal Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis
| ||
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma. | ||
J Am Heart Assoc Paroxetine Attenuates Cardiac Hypertrophy Via Blocking GRK2 and ADRB1 Interaction in Hypertension.
| ||
iScience 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Charlotte (Verified Customer) (07-23-2024) | I tested the antibody as followed: Blocking 1h in 5% milk in TBST 1/1000 in blocking solution, O.N. at 4°C 5*5min TBST 1/5000 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS in IMCD3 ; I obtained GRK2 signal (the middle band out of the 3) but also many aspecific bands. I also tested it in IF since I had it (the ab is not approved for this application) but I got no signal
 |
FH Franziska (Verified Customer) (09-11-2023) | Since there are no GRK-2 antibodies specific for C.elegans, I decided to try this one which didn't work very well, there was a lot of unspecific binding however, C.elegans was not mentioned as tested reactivity.
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