AFM Polyclonal antibody, PBS Only

• 

  WB analysis of human plasma using 19494-1-AP (same clone as 19494-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 19494-1-AP (AFM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19494-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 19494-1-AP (same clone as 19494-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (19494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19494-1-PBS in a different storage buffer formulation.

AFM Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 19494-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

19494-1-PBS targets AFM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: afamin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: NM_001133
• Gene Symbol: AFM
• Gene ID (NCBI): 173
• RRID: AB_2878581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43652
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.