AFM Polyclonal antibody

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• AFM Polyclonal antibody (19494-1-AP)

AFM Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 19494-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Alpha Alb, ALF, ALBA, ALB2, afamin/AFM

• 

  WB analysis of human plasma using 19494-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 19494-1-AP (AFM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 19494-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (19494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human blood tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19494-1-AP targets AFM in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: afamin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: NM_001133
• Gene Symbol: AFM
• Gene ID (NCBI): 173
• RRID: AB_2878581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43652
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

Publications

What published studies show

The AFM Polyclonal antibody (Cat# 19494-1-AP) has a total of 1 citation. It appears in Molecular and Cellular Endocrinology (2016), in a study investigating how Afamin promotes glucose metabolism in papillary thyroid carcinoma. The research field encompasses endocrinology, thyroid cancer, and metabolic regulation. The antibody was used for Western blot in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell Endocrinol; Afamin promotes glucose metabolism in papillary thyroid carcinoma.; Authors - Chen-Tian Shen