AGER/RAGE Polyclonal antibody

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  WB analysis using 32053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32053-1-AP (AGER/RAGE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 32053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32053-1-AP (AGER/RAGE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 32053-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 32053-1-AP (AGER/RAGE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AGER/RAGE Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32053-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AGER, RAGE, Advanced glycosylation end product-specific receptor

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue
• Positive IHC detected in: rat lung tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32053-1-AP targets AGER/RAGE in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGER/RAGE fusion protein Eg1135
• Full Name: advanced glycosylation end product-specific receptor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42-55 kDa
• Gene Symbol: Ager
• Gene ID (NCBI): 81722
• RRID: AB_3670178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q63495
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).