CoraLite® Plus 488-conjugated AIDA Monoclonal antibody

AIDA Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D11A1

Cat no : CL488-68153

Synonyms

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

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Validation Data Gallery

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  IF Staining of A431 using CL488-68153

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 AIDA antibody (CL488-68153, Clone: 1D11A1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68153 targets AIDA in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AIDA fusion protein Ag20597
• Full Name: axin interactor, dorsalization associated
• Calculated Molecular Weight: 306 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC015535
• Gene Symbol: AIDA
• Gene ID (NCBI): 64853
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.