AIFM3 Polyclonal antibody

AIFM3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14778-1-AP

Synonyms

AIFL, AIFM3, Apoptosis inducing factor 3



Tested Applications

Positive WB detected inHEK-293 cells, U-87 MG cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

14778-1-AP targets AIFM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIFM3 fusion protein Ag6278
Full Name apoptosis-inducing factor, mitochondrion-associated, 3
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC032485
Gene symbol AIFM3
Gene ID (NCBI) 150209
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is a mitochondrial protein/flavoenzyme and its gene is located on chromosome 22q11.21. Mature human AIFM3 protein consists of 598 amino acids with a molecular weight of 66 kDa and is predominantly localized in the mitochondria. AIFM3 is widely expressed in many tissues, and breast cancer tissues and cholangiocarcinoma tissue (PMID: 32664187).

Protocols

Product Specific Protocols
WB protocol for AIFM3 antibody 14778-1-APDownload protocol
IHC protocol for AIFM3 antibody 14778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols