AIM2 Polyclonal antibody, PBS Only

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  WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 20590-1-AP (same clone as 20590-1-PBS)

  IP result of anti-AIM2 (IP:20590-1-AP, 4ug; Detection:20590-1-AP 1:600) with Raji cells lysate 2200 ug. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20590-1-AP (same clone as 20590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20590-1-AP (same clone as 20590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 20590-1-AP (same clone as 20590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AIM2 antibody (20590-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

AIM2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 20590-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Interferon-inducible protein AIM2, PYHIN4

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Product Information

20590-1-PBS targets AIM2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14520

  Product name: Recombinant human AIM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-343 aa of BC010940

  Sequence: MESKYKEILLLTGLDNITDEELDRFKFFLSDEFNIATGKLHTANRIQVATLMIQNAGAVSAVMKTIRIFQKLNYMLLAKRLQEEKEKVDKQYKSVTKPKPLSQAEMSPAASAAIRNDVAKQRAAPKVSPHVKPEQKQMVAQQESIREGFQKRCLPVMVLKAKKPFTFETQEGKQEMFHATVATEKEFFFVKVFNTLLKDKFIPKRIIIIARYYRHSGFLEVNSASRVLDAESDQKVNVPLNIIRKAGETPKINTLQTQPLGTIVNGLFVVQKVTEKKKNILFDLSDNTGKMEVLGVRNEDTMKCKEGDKVRLTFFTLSKNGEKLQLTSGVHSTIKVIKAKKKK

• Full Name: absent in melanoma 2
• Calculated Molecular Weight: 343 aa, 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC010940
• Gene Symbol: AIM2
• Gene ID (NCBI): 9447
• RRID: AB_10694420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14862
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria (PMID:22695634). Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity (PMID:16432157,20566831). It has been reported that AIM2 protein levels are significantly elevated in alveolar macrophages of patients with IPF and bleomycin-treated mice (PMID: 38078661). AIM2 inflammasome was also significantly activated, which was also confirmed in Aβ-treated microglial cells (PMID: 34861878)