AIM2 Polyclonal antibody
AIM2 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 20590-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, A549 cells, COLO 320 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse testis tissue, rat testis tissue, SH-SY5Y cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 5 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
The immunogen of 20590-1-AP is AIM2 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AIM2 fusion protein Ag14520 |
Full Name | absent in melanoma 2 |
Calculated molecular weight | 343 aa, 39 kDa |
Observed molecular weight | 39-45 kDa |
GenBank accession number | BC010940 |
Gene symbol | AIM2 |
Gene ID (NCBI) | 9447 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831].
Protocols
Product Specific Protocols | |
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WB protocol for AIM2 antibody 20590-1-AP | Download protocol |
IHC protocol for AIM2 antibody 20590-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Immunol Endoplasmic Reticulum Stress Induces Macrophages to Produce IL-1β During Mycobacterium bovis Infection via a Positive Feedback Loop Between Mitochondrial Damage and Inflammasome Activation. | ||
Neural Regen Res Expression and localization of absent in melanoma 2 in the injured spinal cord. | ||
Hum Immunol Correlation of AIM2 expression in peripheral blood mononuclear cells from humans with acute and chronic hepatitis B. | ||
Inflammation Aspergillus fumigatus-Stimulated Human Corneal Epithelial Cells Induce Pyroptosis of THP-1 Macrophages by Secreting TSLP. | ||
Front Immunol Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. | ||
Mucosal Immunol AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection. |