AIP/ARA9 Polyclonal antibody

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• AIP/ARA9 Polyclonal antibody (18176-1-AP)

AIP/ARA9 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 18176-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

AIP, ARA9, XAP 2, XAP2, AH receptor-interacting protein

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 18176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 18176-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 18176-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 18176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 18176-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug AIP/ARA9 Polyclonal antibody (18176-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18176-1-AP targets AIP/ARA9 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12869

  Product name: Recombinant human AIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-330 aa of BC104797

  Sequence: MADIIARLREDGIQKRVIQEGRGELPDFQDGTKATFHYRTLHSDDEGTVLDDSRARGKPMELIIGKKFKLPVWETIVCTMREGEIAQFLCDIKHVVLYPLVAKSLRNIAVGKDPLEGQRHCCGVAQMREHSSLGHADLDALQQNPQPLIFHMEMLKVESPGTYQQDPWAMTDEEKAKAVPLIHQEGNRLYREGHVKEAAAKYYDAIACLKNLQMKEQPGSPEWIQLDKQITPLLLNYCQCKLVVEEYYEVLDHCSSILNKYDDNVKAYFKRGKAHAAVWNAQEAQADFAKVLELDPALAPVVSRELRALEARIRQKDEEDKARFRGIFSH

• Full Name: aryl hydrocarbon receptor interacting protein
• Calculated Molecular Weight: 330 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC104797
• Gene Symbol: AIP
• Gene ID (NCBI): 9049
• RRID: AB_11182727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00170
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The AIP/ARA9 Polyclonal antibody (Cat# 18176-1-AP) has a total of 2 citations. These appear in Journal of Clinical Investigation and Heliyon. The cited research spans cellular proteostasis, mitochondrial redox balance, and autophagy (mouse, IP), as well as osteogenic differentiation of periodontal ligament stem cells and bone regeneration in periodontitis (rat, WB/IHC).

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IP	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk
rat	WB,IHC	Heliyon; PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats; Authors - Wei Zhang