AKAP1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85119-2-RR (same clone as 85119-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85119-2-RR (AKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85119-2-RR (AKAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85119-2-RR (AKAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85119-2-RR (same clone as 85119-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85119-2-RR (same clone as 85119-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 85119-2-RR (same clone as 85119-2-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.13 ug AKAP1 Recombinant antibody (85119-2-RR, Clone:242763A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85119-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85119-2-RR against Human AKAP1 were performed. The affinity constant is 3.62 nM.

AKAP1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 85119-2-PBS

Clone No.242763A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242763A5, AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa

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Product Information

85119-2-PBS targets AKAP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8037

  Product name: Recombinant human AKAP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 31-351 aa of BC000729

  Sequence: GHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQI

• Full Name: A kinase (PRKA) anchor protein 1
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 149 kDa
• GenBank Accession Number: BC000729
• Gene Symbol: AKAP1
• Gene ID (NCBI): 8165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92667
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.