AKT2 Polyclonal antibody

AKT2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 28113-1-AP

Synonyms

AKT2, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt 2, Protein kinase B beta, RAC BETA, RAC PK beta



Tested Applications

Positive WB detected inNIH/3T3 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28113-1-AP targets AKT2 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT2 fusion protein Ag27005
Full Name v-akt murine thymoma viral oncogene homolog 2
Observed molecular weight56 kDa
GenBank accession numberBC120994
Gene symbol AKT2
Gene ID (NCBI) 208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for AKT2 antibody 28113-1-APDownload protocol
IHC protocol for AKT2 antibody 28113-1-APDownload protocol
IF protocol for AKT2 antibody 28113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols