Albumin Monoclonal antibody

• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of human plasma using 66051-1-Ig

  human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 66051-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66051-1-Ig (ALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 66051-1-Ig

  IP result of anti-Albumin (IP:66051-1-Ig, 4ug; Detection:66051-1-Ig 1:1000) with HepG2 cells lysate 1720 ug.

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  IHC staining of human liver using 66051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 66051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66051-1-Ig (Albumin antibody) at dilution of 1:64000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 66051-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 66051-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66051-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 66051-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 66051-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66051-1-Ig against Human Albumin were performed. The affinity constant is 0.574 nM

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Albumin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 66051-1-Ig

Clone No.4A1C11

Publications(51)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

ALB, 4A1C11, HSA, Serum albumin

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Tested Applications

• Positive WB detected in: human plasma tissue, human liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 19 publications below
• IHC: See 2 publications below
• IF: See 30 publications below
• IP: See 1 publications below

Product Information

66051-1-Ig targets Albumin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Cited Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9885

  Product name: Recombinant human Albumin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 256-609 aa of BC034023

  Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

• Full Name: albumin
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034023
• Gene Symbol: Albumin
• Gene ID (NCBI): 213
• RRID: AB_11042320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02768
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	IF	J Hepatol; Targeting NFATc4 attenuates non-alcoholic steatohepatitis in mice.; Authors - Meng Du
pig	IF	Sci Bull (Beijing); Single-cell dynamics of liver development in postnatal pigs; Authors - Lin Rao
human	WB	J Extracell Vesicles; Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles; Authors - Liqiao Hu
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuan (Verified Customer) (10-15-2019)

This Albumin antibody is working very well on human seminal plasma and blood plasma.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human seminal plasma and blood plasma