CoraLite® Plus 488-conjugated Albumin Monoclonal antibody

Albumin Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (1)

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

4A1C11

Cat no : CL488-66051

Synonyms

ALB, albumin, DKFZp779N1935, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL488-66051

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated Albumin antibody (CL488-66051, Clone: 4A1C11 ) at dilution of 1:400.

• 

  IF Staining of HepG2 using CL488-66051

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Albumin antibody (CL488-66051, Clone: 4A1C11 ) at dilution of 1:200.

• 

  FC experiment of HepG2 using CL488-66051

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Albumin (CL488-66051, Clone:4A1C11) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-66051 targets Albumin in IF, FC (Intra) applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Albumin fusion protein Ag9885
• Full Name: albumin
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034023
• Gene Symbol: ALB
• Gene ID (NCBI): 213
• RRID: AB_2883241
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

Publications

Species	Application	Title
mouse	IF	Mol Cell; WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy.; Authors - Sixian Qi

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C