ALCAM Monoclonal antibody

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  WB analysis using 67768-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67768-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67768-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 67768-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 67768-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse breast cancer using 67768-1-Ig

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of hTERT-RPE1 using 67768-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

ALCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Cat No. 67768-1-Ig

Clone No.1F7D6

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Synonyms

MEMD, CD166, 1F7D6

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Tested Applications

• Positive WB detected in: HT-1080 cells, U2OS cells, A549 cells, LNCaP cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive IF-Fro detected in: mouse breast cancer
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67768-1-Ig targets ALCAM in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALCAM fusion protein Ag16892
• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 583 aa, 65 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC137097
• Gene Symbol: ALCAM
• Gene ID (NCBI): 214
• RRID: AB_2918534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13740
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Publications

Species	Application	Title
human	WB	Antiviral Res; Harnessing host enhancers of SARS-CoV-2 entry as novel targets for antiviral therapy; Authors - Nathalia Williams