ALCAM Monoclonal antibody
ALCAM Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1F7D6
Cat no : 67768-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-1080 cells, U2OS cells, A549 cells, LNCaP cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human breast cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
67768-1-Ig targets ALCAM in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ALCAM fusion protein Ag16892 |
Full Name | activated leukocyte cell adhesion molecule |
Calculated molecular weight | 583 aa, 65 kDa |
Observed molecular weight | 90-100 kDa |
GenBank accession number | BC137097 |
Gene symbol | ALCAM |
Gene ID (NCBI) | 214 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).
Protocols
Product Specific Protocols | |
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WB protocol for ALCAM antibody 67768-1-Ig | Download protocol |
IHC protocol for ALCAM antibody 67768-1-Ig | Download protocol |
IF protocol for ALCAM antibody 67768-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |