ALCAM Monoclonal antibody

ALCAM Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1F7D6

Cat no : 67768-1-Ig

Synonyms

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD



Tested Applications

Positive WB detected inHT-1080 cells, U2OS cells, A549 cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67768-1-Ig targets ALCAM in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALCAM fusion protein Ag16892
Full Name activated leukocyte cell adhesion molecule
Calculated molecular weight 583 aa, 65 kDa
Observed molecular weight90-100 kDa
GenBank accession numberBC137097
Gene symbol ALCAM
Gene ID (NCBI) 214
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Protocols

Product Specific Protocols
WB protocol for ALCAM antibody 67768-1-IgDownload protocol
IHC protocol for ALCAM antibody 67768-1-IgDownload protocol
IF protocol for ALCAM antibody 67768-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols