ALCAM Monoclonal antibody, PBS Only

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  WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

ALCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Cat No. 67768-1-PBS

Clone No.1F7D6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Synonyms

1F7D6, CD166, MEMD

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Product Information

67768-1-PBS targets ALCAM in WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16892

  Product name: Recombinant human ALCAM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 28-400 aa of BC137097

  Sequence: WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVE

• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 583 aa, 65 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC137097
• Gene Symbol: ALCAM
• Gene ID (NCBI): 214
• RRID: AB_2918534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13740
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).