Tested Applications
| Positive WB detected in | 37°C incubated HepG2 cells, HepG2 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | mouse ovary tissue, human placenta tissue, rat cerebellum tissue, rat colon tissue, rat stomach tissue, mouse colon tissue, human kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16675-1-AP targets AMFR/GP78 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10178 Product name: Recombinant human AMFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 301-643 aa of BC069197 Sequence: LFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS Predict reactive species |
| Full Name | autocrine motility factor receptor |
| Calculated Molecular Weight | 643 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC069197 |
| Gene Symbol | AMFR/GP78 |
| Gene ID (NCBI) | 267 |
| RRID | AB_2226463 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKV5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| IF protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| IHC protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| IP protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| WB protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The gp78/AMFR/INSIG1 antibody (Cat# 16675-1-AP) has 52 citations across journals including Nature, Cell, Immunity, Nature Communications, EMBO Journal, Cell Death & Differentiation, PLoS Biology, and eLife. Research fields span ER-phagy, ERAD, ubiquitin-mediated protein degradation, apoptosis, ferroptosis, inflammation, cancer (hepatocellular, prostate, breast, colorectal, glioblastoma), cardiovascular disease, lipid metabolism, viral pathogenesis, neurodegeneration, and circadian biology. Applications include WB, IF, IHC, CoIP, and IP.
| Species | Application | Title |
|---|---|---|
Cell BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
| ||
J Hepatol eIF3f promotes tumour malignancy by remodelling fatty acid biosynthesis in hepatocellular carcinoma | ||
Immunity The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING.
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Reviews
What customers say
Users report this antibody performs excellently for Western blotting and immunoprecipitation, with strong specificity validated in knockout lines. One reviewer at 1:2000 dilution called it "one of the best" for Gp78 WB on human cancer cell lines. Another noted it works well across multiple cell lines and tissues for WB and IP, but is less ideal for immunofluorescence or ICC. A minor concern raised was the appearance of non-specific bands that may increase as the antibody ages. Overall, it is well-regarded for WB and IP applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bharat (Verified Customer) (03-19-2026) | One of the best and very specific antibody for the Gp78 western blotting.
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Bharat (Verified Customer) (10-02-2025) | Excellent in general for WB and IP and we have validated the specificity in knockout line. Not that great for ICC or IF. Although, we do some some non specific bands and over time, it increases as antibody gets old.
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