- Featured Product
- KD/KO Validated
AMFR/GP78 Polyclonal antibody
AMFR/GP78 Polyclonal Antibody for FC, IF, IP, WB, ELISA
Cat no : 16675-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, 37°C incubated HepG2 cells, MCF-7 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16675-1-AP targets AMFR/GP78 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AMFR/GP78 fusion protein Ag10178|
|Full Name||autocrine motility factor receptor|
|Calculated molecular weight||643 aa, 73 kDa|
|Observed molecular weight||73 kDa|
|GenBank accession number||BC069197|
|Gene ID (NCBI)||267|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).
|Product Specific Protocols|
|WB protocol for AMFR/GP78 antibody 16675-1-AP||Download protocol|
|IHC protocol for AMFR/GP78 antibody 16675-1-AP||Download protocol|
|IF protocol for AMFR/GP78 antibody 16675-1-AP||Download protocol|
|IP protocol for AMFR/GP78 antibody 16675-1-AP||Download protocol|
|Click here to view our Standard Protocols|
BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING.
Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.
MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.
Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.
The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.