AMHR2 Polyclonal antibody

AMHR2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25888-1-AP

Synonyms

AMH type II receptor, AMHR, AMHR2, MIS type II receptor, MISR2, MISRII, MRII

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Validation Data Gallery

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  IHC staining of human testis using 25888-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25888-1-AP (AMHR2 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 25888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25888-1-AP (AMHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25888-1-AP (AMHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 25888-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25888-1-AP (AMHR2 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 25888-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25888-1-AP (AMHR2 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 25888-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25888-1-AP (AMHR2 Antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive IHC detected in: human testis tissue, human ovary tumor tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25888-1-AP targets AMHR2 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMHR2 fusion protein Ag22473
• Full Name: anti-Mullerian hormone receptor, type II
• Calculated Molecular Weight: 573 aa, 63 kDa
• GenBank Accession Number: BC126316
• Gene Symbol: AMHR2
• Gene ID (NCBI): 269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-Mullerian hormone (Amh) is the key factor in the regression of the Mullerian ducts that is secreted by the Sertoli cells of the testis. As a member of the transforming growth factor β family, Amh signals through a heterodimeric receptor complex consisting of two type I and two types II serine/threonine kinases receptors (J. Klatting, et. al, 2007). On ligand binding, Type II receptors phosphorylate and activate Type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. The Amh type II receptor (Amhr2) confers the specificity and is initially expressed in the coelomic epithelium next to the Mullerian ducts in both males and females (Zhan, y. et. al, 2006).