Tested Applications
| Positive IHC detected in | human testis tissue, human ovary tumor tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25888-1-AP targets AMHR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22473 Product name: Recombinant human AMHR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 226-573 aa of BC126316 Sequence: LVAIKAFPPRSVAQFQAERALYELPGLQHDHIVRFITASRGGPGRLLSGPLLVLELHPKGSLCHYLTQYTSDWGSSLRMALSLAQGLAFLHEERWQNGQYKPGIAHRDLSSQNVLIREDGSCAIGDLGLALVLPGLTQPPAWTPTQPQGPAAIMEAGTQRYMAPELLDKTLDLQDWGMALRRADIYSLALLLWEILSRCPDLRPDSSPPPFQLAYEAELGNTPTSDELWALAVQERRRPYIPSTWRCFATDPDGLRELLEDCWDADPEARLTAECVQQRLAALAHPQESHPFPESCPRGCPPLCPEDCTSIPAPTILPCRPQRSACHFSVQQGPCSRNPQPACTLSPV Predict reactive species |
| Full Name | anti-Mullerian hormone receptor, type II |
| Calculated Molecular Weight | 573 aa, 63 kDa |
| GenBank Accession Number | BC126316 |
| Gene Symbol | AMHR2 |
| Gene ID (NCBI) | 269 |
| RRID | AB_3085826 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Anti-Mullerian hormone (AMH) is the key factor in the regression of the Müllerian ducts and is secreted by Sertoli cells of the testis. As a member of the transforming growth factor β family, Amh signals through a heterodimeric receptor complex consisting of two type I and two type II serine/threonine kinase receptors (PMID: 18391537). On ligand binding, Type II receptors phosphorylate and activate Type I receptors, which autophosphorylate, then bind and activate SMAD transcriptional regulators. The Amh type II receptor (Amhr2) confers the specificity and is initially expressed in the coelomic epithelium next to the Müllerian ducts in both males and females (PMID: 34515230).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AMHR2 antibody 25888-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AMHR2 Polyclonal antibody (Cat# 25888-1-AP) has 1 total citation. It appears in the Journal of Assisted Reproduction and Genetics, a publication focused on reproductive biology. The cited study investigated a novel anti-Müllerian hormone receptor 2 binding peptide for preventing chemotherapy-related ovarian follicle loss in a mouse model, placing the research in the fields of reproductive medicine and ovarian toxicology.
| Species | Application | Title |
|---|---|---|
J Assist Reprod Genet Novel anti-Mullerian hormone receptor 2 binding peptide prevents chemotherapy-related ovarian follicle loss in a mouse model. |
