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ANK1 Polyclonal antibody

ANK1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23938-1-AP

Synonyms

ANK, ANK 1, ANK1, Ankyrin 1, ankyrin 1, erythrocytic, Ankyrin R, Erythrocyte ankyrin, SPH1, SPH2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANK1 Polyclonal antibody (23938-1-AP)

    WB analysis using 23938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23938-1-AP (ANK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23938-1-AP targets ANK1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANK1 fusion protein Ag21041
Full Name ankyrin 1, erythrocytic
Calculated Molecular Weight 155 aa, 18 kDa
Observed Molecular Weight 190-250 kDa
GenBank Accession NumberBC030957
Gene Symbol ANK1
Gene ID (NCBI) 286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ankyrin-1 (ANK1) is also named as Ankyrin-R and Erythrocyte ankyrin. The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex (PMID:18440852). ANK1 can attache integral membrane proteins to cytoskeletal elements. And it binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin.(PMID:12456646).

Protocols

Product Specific Protocols
WB protocol for ANK1 antibody 23938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols