ANK1 Polyclonal antibody

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  WB analysis using 23938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23938-1-AP (ANK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 23938-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 23938-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 23938-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 23938-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 23938-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 23938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANK1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23938-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Ankyrin 1, ANK, ANK 1, ANK-1, Ankyrin R

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Tested Applications

• Positive WB detected in: K-562 cells, mouse liver tissue
• Positive IHC detected in: human stomach cancer tissue, human lymphoma tissue, mouse cerebellum tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23938-1-AP targets ANK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21041

  Product name: Recombinant human ANK1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-155 aa of BC030957

  Sequence: MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

• Full Name: ankyrin 1, erythrocytic
• Calculated Molecular Weight: 155 aa, 18 kDa
• Observed Molecular Weight: 190-250 kDa
• GenBank Accession Number: BC030957
• Gene Symbol: ANK1
• Gene ID (NCBI): 286
• RRID: AB_3085717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P16157-17
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ankyrin-1 (ANK1) is also named as Ankyrin-R and Erythrocyte ankyrin. The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex (PMID:18440852). ANK1 can attache integral membrane proteins to cytoskeletal elements. And it binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin.(PMID:12456646). This target recognizes the C-terminal of Ankyrin-1 and also recognizes ankyrin-1 isoform 5 (206 kDa). It can identify isoforms such as Er5, Er6, Er11, Er12, Er13, Er14, Er16, Mu17, Mu18, Mu19, Mu20, 22, and 23.