ANKIB1 Polyclonal antibody

ANKIB1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30467-1-AP

Synonyms

ANKIB1, DKFZp434A0225, FLJ33123, KIAA1386

---

Validation Data Gallery

• 

  WB analysis using 30467-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30467-1-AP (ANKIB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 30467-1-AP

  IP result of anti-ANKIB1 (IP:30467-1-AP, 4ug; Detection:30467-1-AP 1:4000) with HeLa cells lysate 1560 ug.

• 

  IHC staining of mouse kidney using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 30467-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 30467-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse kidney tissue, mouse placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30467-1-AP targets ANKIB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKIB1 fusion protein Ag33126
• Full Name: ankyrin repeat and IBR domain containing 1
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 122 kDa
• GenBank Accession Number: NM_019004.1
• Gene Symbol: ANKIB1
• Gene ID (NCBI): 54467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ankyrin repeat and IBR domain-containing protein 1 (ANKIB1) is also named as KIAA1386, and belongs to the RBR family. ANKIB1 might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. The common biomarkers found within the H and EC region of brain are ZNF621, SLC25A46, RAE1, and ANKIB1. Among these biomarkers, RAE1, ANKIB1, and SLC25A46 have been reported to be prominently involved in several neurodegenerative disorders (PMID:33878365). ANKIB1 is also found to be associated with Cerebral cavernous malformations (PMID:20798775).