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ANP32B Monoclonal antibody

ANP32B Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat







Cat no : 66160-1-Ig


ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

Tested Applications

Positive WB detected inLNCaP cells, fetal human brain tissue, HEK-293 cells, mouse brain tissue, U2OS cells, A431 cells, HCT 116 cells, HeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66160-1-Ig targets ANP32B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ANP32B fusion protein Ag1274
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC013003
Gene Symbol ANP32B
Gene ID (NCBI) 10541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).


Product Specific Protocols
WB protocol for ANP32B antibody 66160-1-IgDownload protocol
IF protocol for ANP32B antibody 66160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Virol

ANP32 proteins are essential for influenza virus replication in human cells.

Authors - Ecco Staller
  • KO Validated