CoraLite®594-conjugated ANP32B Monoclonal antibody

ANP32B Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A1F12

Cat no : CL594-66160

Synonyms

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66160-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66160 targets ANP32B in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ANP32B fusion protein Ag1274
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Calculated molecular weight 29 kDa
GenBank accession numberBC013003
Gene symbol ANP32B
Gene ID (NCBI) 10541
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Protocols

Product Specific Protocols
IF protocol for CL594 ANP32B antibody CL594-66160Download protocol
Standard Protocols
Click here to view our Standard Protocols