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Annexin A2 Monoclonal antibody
Annexin A2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 66035-1-Ig
Validation Data Gallery
|Positive WB detected in
|K-562 cells, MCF-7 cells, human brain tissue, HepG2 cells, HEK-293 cells, A549 cells, HeLa cells, A431 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
|Positive IHC detected in
|human breast cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|T-47D cells, HeLa cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:5000-1:20000
|IF : 1:250-1:1000
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66035-1-Ig targets Annexin A2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Mouse / IgG2a
|Annexin A2 fusion protein Ag16654
|Calculated molecular weight
|339 aa, 39 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
Hypoxia-induced circADAMTS6 in a TDP43-dependent manner accelerates glioblastoma progression via ANXA2/ NF-κB pathway
Chip-DSF: A rapid screening strategy for drug protein targets.
J Exp Clin Cancer Res
Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma.
Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.
Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells.
An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.