Annexin A2 Monoclonal antibody

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  WB analysis using 66035-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66035-1-Ig

  WB result of Annexin A2 antibody (66035-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A2 transfected HEK-293 cells.

• 

  WB analysis using 66035-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66035-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66035-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66035-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of T-47D using 66035-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66035-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66035-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin A2 Monoclonal antibody (66035-1-Ig, Clone:1C1E12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66035-1-Ig

Clone No.1C1E12

Publications(23)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells, human brain tissue, HepG2 cells, HEK-293 cells, A549 cells, HeLa cells, A431 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: T-47D cells, HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 10 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below

Product Information

66035-1-Ig targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16654

  Product name: Recombinant human Annexin A2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-339 aa of BC016774

  Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

• Full Name: annexin A2
• Calculated Molecular Weight: 339 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC016774
• Gene Symbol: Annexin A2
• Gene ID (NCBI): 302
• ENSEMBL Gene ID: ENSG00000182718
• RRID: AB_11045659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07355
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF,IP	Oncogene; Hypoxia-induced circADAMTS6 in a TDP43-dependent manner accelerates glioblastoma progression via ANXA2/ NF-κB pathway; Authors - Shulin Zhao ; KD Validated
human		Pharmacol Res; Chip-DSF: A rapid screening strategy for drug protein targets.; Authors - Zhao Cui
human	WB, IF, IP	J Exp Clin Cancer Res; Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma.; Authors - Lu Deng
human	WB,IHC,IF,CoIP	Biomed Pharmacother; Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.; Authors - Lei Wang
human	WB	Biosci Rep; Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells.; Authors - Jing Gu ; KD Validated
		Analyst; An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.; Authors - Yu Yanyan Y