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Annexin A2 Monoclonal antibody
Annexin A2 Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Cat no : 66035-1-Ig
|Positive WB detected in||K-562 cells, HepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, A549 cells, HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||T-47D cells, HeLa cells|
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:5000-1:20000|
|Immunofluorescence (IF)||IF : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66035-1-Ig is Annexin A2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Annexin A2 fusion protein Ag16654|
|Full Name||annexin A2|
|Calculated molecular weight||339 aa, 39 kDa|
|Observed molecular weight||39 kDa|
|GenBank accession number||BC016774|
|Gene ID (NCBI)||302|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
|Product Specific Protocols|
|WB protocol for Annexin A2 antibody 66035-1-Ig||Download protocol|
|IHC protocol for Annexin A2 antibody 66035-1-Ig||Download protocol|
|IF protocol for Annexin A2 antibody 66035-1-Ig||Download protocol|
|FC protocol for Annexin A2 antibody 66035-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells.
Cell Death Dis
TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy.
Mol Med Rep
Human epididymis protein 4 promotes P‑glycoprotein‑mediated chemoresistance in ovarian cancer cells through interactions with Annexin II.
Cancer Cell Int
Interaction of HE4 and ANXA2 exists in various malignant cells-HE4-ANXA2-MMP2 protein complex promotes cell migration.
An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.
Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.