• Featured Product
  • KD/KO Validated

Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

CloneNo.

1C1E12

Cat no : 66035-1-Ig

Synonyms

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I



Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, A549 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inT-47D cells, HeLa cells
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66035-1-Ig is Annexin A2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Annexin A2 fusion protein Ag16654
Full Name annexin A2
Calculated molecular weight 339 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC016774
Gene symbol ANXA2
Gene ID (NCBI) 302
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Protocols

Product Specific Protocols
WB protocol for Annexin A2 antibody 66035-1-IgDownload protocol
IHC protocol for Annexin A2 antibody 66035-1-IgDownload protocol
IF protocol for Annexin A2 antibody 66035-1-IgDownload protocol
FC protocol for Annexin A2 antibody 66035-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biosci Rep

Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells.

Authors - Jing Gu
  • KD Validated
humanWB,IF

Cell Death Dis

TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy.

Authors - Huan-Tian Zhang
humanWB, CoIP, IF

Mol Med Rep

Human epididymis protein 4 promotes P‑glycoprotein‑mediated chemoresistance in ovarian cancer cells through interactions with Annexin II.

Authors - Qing Liu
humanWB,IHC,IF,CoIP

Cancer Cell Int

Interaction of HE4 and ANXA2 exists in various malignant cells-HE4-ANXA2-MMP2 protein complex promotes cell migration.

Authors - Jing Wang

Analyst

An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.

Authors - Yu Yanyan Y
human,mouseWB,IHC,IF,CoIP

Biomed Pharmacother

Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.

Authors - Lei Wang