- Featured Product
- KD/KO Validated
Annexin A2 Monoclonal antibody
Annexin A2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
human, mouse, rat
WB, IP, IHC, IF, FC, CoIP, ELISA
Cat no : 66035-1-Ig
Validation Data Gallery
|Positive WB detected in||K-562 cells, MCF-7 cells, human brain tissue, HepG2 cells, HEK-293 cells, A549 cells, HeLa cells, A431 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells|
|Positive IHC detected in||human breast cancer tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||T-47D cells, HeLa cells|
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:5000-1:20000|
|Immunofluorescence (IF)||IF : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 10 publications below|
|IHC||See 3 publications below|
|IF||See 7 publications below|
|IP||See 2 publications below|
|CoIP||See 3 publications below|
66035-1-Ig targets Annexin A2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Annexin A2 fusion protein Ag16654|
|Full Name||annexin A2|
|Calculated molecular weight||339 aa, 39 kDa|
|Observed molecular weight||39 kDa|
|GenBank accession number||BC016774|
|Gene ID (NCBI)||302|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
|Product Specific Protocols|
|WB protocol for Annexin A2 antibody 66035-1-Ig||Download protocol|
|IHC protocol for Annexin A2 antibody 66035-1-Ig||Download protocol|
|IF protocol for Annexin A2 antibody 66035-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Hypoxia-induced circADAMTS6 in a TDP43-dependent manner accelerates glioblastoma progression via ANXA2/ NF-κB pathway
Chip-DSF: A rapid screening strategy for drug protein targets.
J Exp Clin Cancer Res
Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma.
Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.
An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.
Cancer Cell Int
Interaction of HE4 and ANXA2 exists in various malignant cells-HE4-ANXA2-MMP2 protein complex promotes cell migration.