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Annexin VII Polyclonal antibody

Annexin VII Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10154-2-AP

Synonyms

Annexin 7, annexin A7, Annexin VII, Annexin7, ANX7, ANXA7, SNX, SYNEXIN



Tested Applications

Positive WB detected inmouse heart tissue, Jurkat cells, L02 cells, mouse brain tissue, mouse lung tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman pancreas tissue, human colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10154-2-AP targets Annexin VII in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin VII fusion protein Ag0206
Full Name annexin A7
Calculated molecular weight 50 kDa
Observed molecular weight 47 kDa, 51 kDa
GenBank accession numberBC002632
Gene symbol ANXA7
Gene ID (NCBI) 310
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A7 (Anx7) belongs to a ubiquitous and relatively abundant family of Ca2+-dependent membrane-binding proteins, which are thought to be involved in multiple aspects of cell biology including membrane trafficking, mediation of cell-matrix interactions and membrane organization within cells. Anx7, migrated as a 50 kDa protein in SDS-PAGE, has been proposed to function in the fusion of vesicles, acting as a Ca++ channel and as Ca++ -activated GTPase, thus inducing Ca++ /GTP-dependent secretory events.

Protocols

Product Specific Protocols
WB protocol for Annexin VII antibody 10154-2-APDownload protocol
IHC protocol for Annexin VII antibody 10154-2-APDownload protocol
IP protocol for Annexin VII antibody 10154-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Transl Res

Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression.

Authors - Hu-Fang Yuan
humanIHC

Int J Clin Exp Pathol

Inhibitory effect of siRNA-Annexin A7 on growth, migration, and invasion in BGC823 cells and gastric cancer xenograftsin nude mice.

Authors - Hu-Fang Yuan
humanWB

Oncol Lett

Annexin A7 expression is downregulated in late-stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate.

Authors - Weihua Ye
humanWB,IHC

Int J Clin Exp Pathol

Expression of annexin A7 and its clinical significance in differentiation and metastasis of gastric carcinoma.

Authors - Hu-Fang Yuan
humanWB

Biomed Pharmacother

Ezrin expression is altered in mice lymphatic metastatic hepatocellular carcinoma and subcellular fractions upon Annexin 7 modulation in-vitro.

Authors - Ahmed Musa Hago
humanWB

Mol Cell Biochem

Effect of annexin A7 suppression on the apoptosis of gastric cancer cells.

Authors - Weihua Ye
  • KD Validated