Validation Data Gallery
|Positive WB detected in||A549 cells, Jurkat cells, mouse lung tissue, L02 cells, mouse brain tissue, mouse heart tissue, HeLa cells, RAW 264.7 cells, rat brain tissue|
|Positive IP detected in||U-87 MG cells, mouse heart tissue|
|Positive IHC detected in||human pancreas tissue, human prostate cancer tissue, human stomach tissue, human stomach cancer tissue, rat stomach tissue, mouse stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:16000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10154-2-AP targets Annexin A7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin A7 fusion protein Ag0206|
|Full Name||annexin A7|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||47 kDa, 51 kDa|
|GenBank accession number||BC002632|
|Gene ID (NCBI)||310|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Annexin A7 (Anx7) belongs to a ubiquitous and relatively abundant family of Ca2+-dependent membrane-binding proteins, which are thought to be involved in multiple aspects of cell biology including membrane trafficking, mediation of cell-matrix interactions and membrane organization within cells. Anx7, migrated as a 50 kDa protein in SDS-PAGE, has been proposed to function in the fusion of vesicles, acting as a Ca++ channel and as Ca++ -activated GTPase, thus inducing Ca++ /GTP-dependent secretory events.
|Product Specific Protocols|
|WB protocol for Annexin A7 antibody 10154-2-AP||Download protocol|
|IHC protocol for Annexin A7 antibody 10154-2-AP||Download protocol|
|IP protocol for Annexin A7 antibody 10154-2-AP||Download protocol|
|FC protocol for Annexin A7 antibody 10154-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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