Gamma Adaptin Polyclonal antibody

Gamma Adaptin Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13258-1-AP

Synonyms

ADTG, AP 1 complex subunit gamma 1, AP1G1, CLAPG1, Gamma1 adaptin

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 13258-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13258-1-AP (AP1G1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13258-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13258-1-AP (AP1G1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13258-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13258-1-AP (Gamma adaptin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13258-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13258-1-AP (Gamma adaptin Antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse skeletal muscle tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

13258-1-AP targets Gamma Adaptin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Adaptin fusion protein Ag3696
• Full Name: adaptor-related protein complex 1, gamma 1 subunit
• Calculated molecular weight: 825 aa, 92 kDa
• Observed molecular weight: 91 kDa
• GenBank accession number: BC036283
• Gene symbol: AP1G1
• Gene ID (NCBI): 164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	IF	Proc Natl Acad Sci U S A; An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.; Authors - Yan Huang
mouse	WB	Front Cell Neurosci; MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking.; Authors - Xin-Tai Wang
human	IF	Front Cell Dev Biol; Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging.; Authors - Yan Huang