Gamma Adaptin Polyclonal antibody

Gamma Adaptin Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13258-1-AP

Synonyms

ADTG, AP 1 complex subunit gamma 1, AP1G1, CLAPG1, Gamma1 adaptin



Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13258-1-AP targets Gamma Adaptin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma Adaptin fusion protein Ag3696
Full Name adaptor-related protein complex 1, gamma 1 subunit
Calculated molecular weight 825 aa, 92 kDa
Observed molecular weight 91 kDa
GenBank accession numberBC036283
Gene symbol AP1G1
Gene ID (NCBI) 164
RRIDAB_2058209
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Gamma Adaptin antibody 13258-1-APDownload protocol
IHC protocol for Gamma Adaptin antibody 13258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.

Authors - Boris Simonetti
humanIF

Proc Natl Acad Sci U S A

An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.

Authors - Yan Huang
humanIF

Front Cell Dev Biol

Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging.

Authors - Yan Huang
humanWB

Cells

Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells

Authors - Jiahe Lu
mouseWB

Front Cell Neurosci

MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking.

Authors - Xin-Tai Wang
humanWB

bioRxiv

Five inhibitory receptors display distinct vesicular distributions in T cells

Authors - Jiahe Lu