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AP3M1 Polyclonal antibody

AP3M1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 12114-1-AP

Synonyms

AP 3 complex subunit mu 1, AP3M1, Mu adaptin 3A, Mu3A adaptin



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IP detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12114-1-AP targets AP3M1 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3M1 fusion protein Ag2759
Full Name adaptor-related protein complex 3, mu 1 subunit
Calculated molecular weight 418 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC026232
Gene symbol AP3M1
Gene ID (NCBI) 26985
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Protocols

Product Specific Protocols
WB protocol for AP3M1 antibody 12114-1-APDownload protocol
IP protocol for AP3M1 antibody 12114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network.

Authors - Guo Yusong Y
  • KD Validated