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  • KD/KO Validated

AP3M1 Polyclonal antibody

AP3M1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 12114-1-AP


AP 3 complex subunit mu 1, AP3M1, Mu adaptin 3A, Mu3A adaptin

Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12114-1-AP targets AP3M1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3M1 fusion protein Ag2759
Full Name adaptor-related protein complex 3, mu 1 subunit
Calculated Molecular Weight 418 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC026232
Gene Symbol AP3M1
Gene ID (NCBI) 26985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.


Product Specific Protocols
WB protocol for AP3M1 antibody 12114-1-APDownload protocol
IHC protocol for AP3M1 antibody 12114-1-APDownload protocol
IF protocol for AP3M1 antibody 12114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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