|Positive WB detected in||HEK-293 cells, NIH/3T3 cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12114-1-AP targets AP3M1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AP3M1 fusion protein Ag2759|
|Full Name||adaptor-related protein complex 3, mu 1 subunit|
|Calculated molecular weight||418 aa, 47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC026232|
|Gene ID (NCBI)||26985|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network.
Zhachong Shisanwei Pill resists ischemic stroke by lysosome pathway based on proteomics and bioinformatics