APC4 Polyclonal antibody, PBS Only

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  WB analysis using 14129-1-AP (same clone as 14129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14129-1-AP (APC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14129-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 14129-1-AP (same clone as 14129-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14129-1-AP (APC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14129-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14129-1-AP (same clone as 14129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14129-1-AP (APC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14129-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14129-1-AP (same clone as 14129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14129-1-AP (APC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14129-1-PBS in a different storage buffer formulation.

APC4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14129-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

14129-1-PBS targets APC4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5225

  Product name: Recombinant human ANAPC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-343 aa of BC059383

  Sequence: MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTCLAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLPKLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCLSSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSS

• Full Name: anaphase promoting complex subunit 4
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 92 kDa, 63 kDa
• GenBank Accession Number: BC059383
• Gene Symbol: APC4
• Gene ID (NCBI): 29945
• RRID: AB_10640671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJX5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.