Apelin Polyclonal antibody

Apelin Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11497-1-AP

Synonyms

APEL, apelin, APJ endogenous ligand, APLN, XNPEP2



Tested Applications

Positive IHC detected inhuman colon cancer tissue, mouse heart tissue, mouse brain tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

11497-1-AP targets Apelin in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apelin fusion protein Ag2047
Full Name apelin
Calculated Molecular Weight 77 aa, 9 kDa
GenBank Accession NumberBC021104
Gene Symbol APLN
Gene ID (NCBI) 8862
RRIDAB_2877771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apelin, isolated from bovine stomach tissue extractsis, is recognized as the endogenous ligand of the angiotensin-like-receptor 1 (APJ), and the human orphan G-protein-coupled receptor. Apelin is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma. Apelin has been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system. Apelin is also known to be involved in the maintenance of INS sensitivity and play an important role in liver disease.

Protocols

Product Specific Protocols
IHC protocol for Apelin antibody 11497-1-APDownload protocol
IF protocol for Apelin antibody 11497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Sci Adv

Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.

Authors - Jun Seok Son
ratIHC

Free Radic Biol Med

Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway

Authors - Liang Chen
mouse

EBioMedicine

Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.

Authors - Jun Seok Son
rat

Front Pharmacol

Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer's Disease Through Activation of BDNF-TrkB Signaling Pathway.

Authors - Huaiqing Luo
rat

Neuroscience

Apelin-13 Upregulates BDNF Against Chronic Stress-induced Depression-like Phenotypes by Ameliorating HPA Axis and Hippocampal Glucocorticoid Receptor Dysfunctions.

Authors - Ting-Ting Dai
rat

Neuroscience

The Hippocampus is a Critical Site Mediating Antidepressant-like Activity of Apelin-13 in Rats.

Authors - Zhi-Yong Xiao