AQP2 Recombinant antibody

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  WB analysis using 85402-1-RR

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 85402-1-RR (AQP2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85402-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85402-1-RR against Human AQP2 were performed. The affinity constant is 0.955 nM.

AQP2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85402-1-RR

Clone No.242886B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Aquaporin 2, AQP-2, Aquaporin2

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Tested Applications

• Positive WB detected in: mouse kidney tissue, unboiled mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85402-1-RR targets AQP2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AQP2 fusion protein Ag29931
• Full Name: aquaporin 2 (collecting duct)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 28-29, 35-38 kDa
• GenBank Accession Number: NM_000486
• Gene Symbol: AQP2
• Gene ID (NCBI): 359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41181
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.