AQP2 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85402-1-RR (same clone as 85402-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 85402-1-RR (AQP2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85402-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01925-2

  Cytometric bead array standard curve of MP01925-2, AQP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85402-1-PBS. Detection antibody: 85402-3-PBS. Standard: Ag29931. Range: 1.563-200 ng/mL.

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  Cytometric bead array standard curve of MP01925-1

  Cytometric bead array standard curve of MP01925-1, AQP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85402-1-PBS. Detection antibody: 85402-2-PBS. Standard: Ag29931. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 85402-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85402-1-RR against Human AQP2 were performed. The affinity constant is 0.955 nM.

AQP2 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 85402-1-PBS

Clone No.242886B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

Aquaporin 2, AQP-2, Aquaporin2

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Product Information

85402-1-PBS targets AQP2 as part of a matched antibody pair:

MP01925-1: 85402-1-PBS capture and 85402-2-PBS detection (validated in Cytometric bead array)

MP01925-2: 85402-1-PBS capture and 85402-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AQP2 fusion protein Ag29931
• Full Name: aquaporin 2 (collecting duct)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 28-29, 35-38 kDa
• GenBank Accession Number: NM_000486
• Gene Symbol: AQP2
• Gene ID (NCBI): 359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41181
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.